http://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&feed=atom&action=historyTeam:Imperial College London/Data - Revision history2024-03-29T06:20:19ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=255783&oldid=prevRebekkaBauer at 20:37, 28 October 20112011-10-28T20:37:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105" target="_blank"><b>Main Page</b></a> - <b>Composite J23100 promoter - sfGFP, BBa_K515105</b>: We expressed sfGFP and studied its thermostability. We also used it on the confocal microscope to image the uptake of <i>E. coli</i> into the roots.</li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105" target="_blank"><b>Main Page</b></a> - <b>Composite J23100 promoter - sfGFP, BBa_K515105</b>: We expressed sfGFP and studied its thermostability. We also used it on the confocal microscope to image the uptake of <i>E. coli</i> into the roots.</li> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515002" target="_blank"><b>Main Page</b></a> - <b>PA2652, BBa_K515002</b>: We did numerous assays to see whether the bacteria respond to malate. Our first behavioural analysis <del class="diffchange diffchange-inline">seems to indicate </del>that there is an increase in tumbling frequency when malate is present and the cells are expressing PA2652.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515002" target="_blank"><b>Main Page</b></a> - <b>PA2652, BBa_K515002</b>: We did numerous assays to see whether the bacteria respond to malate. Our first behavioural analysis <ins class="diffchange diffchange-inline">showed </ins>that there is an increase in tumbling frequency when malate is present and the cells are expressing PA2652<ins class="diffchange diffchange-inline">. Chemotaxis towards malate was confirmed in a capillary-based assay</ins>.</li></div></td></tr>
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</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=238705&oldid=prevYuanwei at 20:35, 18 October 20112011-10-18T20:35:04Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><i>Figure 1: Schematic diagram summarising our system. It consists of three modules: a chemotaxis module (<a href="https://2011.igem.org/Team:Imperial_College_London/Project_Chemotaxis_Overview"><b>Phyto-Route</b></a>), an auxin-production module (<a href="https://2011.igem.org/Team:Imperial_College_London/Project_Auxin_Overview"><b>Auxin Xpress</b></a>) and (<a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview"><b>Gene Guard</b></a>), a containment module (Diagram by Imperial College London iGEM team 2011). </i></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><i>Figure 1: Schematic diagram summarising our system. It consists of three modules: a chemotaxis module (<a href="https://2011.igem.org/Team:Imperial_College_London/Project_Chemotaxis_Overview"><b>Phyto-Route</b></a>), an auxin-production module (<a href="https://2011.igem.org/Team:Imperial_College_London/Project_Auxin_Overview"><b>Auxin Xpress</b></a>) and (<a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview"><b>Gene Guard</b></a>), a containment module (Diagram by Imperial College London iGEM team 2011). </i></p></div></td></tr>
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</table>Yuanweihttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=238673&oldid=prevNk2909 at 18:36, 18 October 20112011-10-18T18:36:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515100" target="_blank"><b>Main Page</b></a> - <b>Composite pVeg2 - IaaM - IaaH, BBa_K515100</b>: We have shown that indole 3-acetic acid is being produced in our bacteria. In addition, we have exposed a mutant of <i>Arabidopsis</i> that expresses YFP in response to IAA to our auxin-producing bacteria. These plants were fluorescing more brightly than the controls.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515100" target="_blank"><b>Main Page</b></a> - <b>Composite pVeg2 - IaaM - IaaH, BBa_K515100</b>: We have shown that indole 3-acetic acid is being produced in our bacteria. In addition, we have exposed a mutant of <i>Arabidopsis</i> that expresses YFP in response to IAA to our auxin-producing bacteria. These plants were fluorescing more brightly than the controls.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K515107" target="_blank"><b>Main Page</b></a> - <b>Composite p(tetR) R0040- RBS B0034- Dendra2, BBa_K515107</b>: We have successfully expressed and photoconverted this protein in <i>E. coli</i> cells. In addition, we have photoconverted the protein inside bacterial cells that had been taken up into <i>Arabidopsis</i> roots using a confocal microscope.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K515107" target="_blank"><b>Main Page</b></a> - <b>Composite p(tetR) R0040- RBS B0034- Dendra2, BBa_K515107</b>: We have successfully expressed and photoconverted this protein in <i>E. coli</i> cells. In addition, we have photoconverted the protein inside bacterial cells that had been taken up into <i>Arabidopsis</i> roots using a confocal microscope.</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515102" target="_blank"><b>Main Page</b></a> - <b>Composite J23100 promoter - PA2652, BBa_K515102</b>: We <del class="diffchange diffchange-inline">did numerous assays to see whether the </del>bacteria respond to <del class="diffchange diffchange-inline">malate. Our first behavioural analysis seems to indicate that cells expressing construct PA2652 show a uniform response when exposed to </del>L (-) malic acid in comparison to cells that do not contain the construct.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515102" target="_blank"><b>Main Page</b></a> - <b>Composite J23100 promoter - PA2652, BBa_K515102</b>: We <ins class="diffchange diffchange-inline">have shown that our </ins>bacteria respond to <ins class="diffchange diffchange-inline"> </ins>L (-) malic acid <ins class="diffchange diffchange-inline">through chemotactic behavior </ins>in comparison to cells that do not contain the construct<ins class="diffchange diffchange-inline">. We have also quantified this response using capillary assay</ins>.</li></div></td></tr>
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</table>Nk2909http://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=168358&oldid=prevRebekkaBauer at 03:30, 22 September 20112011-09-22T03:30:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515000" target="_blank"><b>Main Page</b></a> - <b>IaaM - tryptophan-2-mono-oxygenase, BBa_K515000</b>: We have shown that indole 3-acetic acid is being produced in our bacteria. In addition, we have exposed a mutant of <i>Arabidopsis</i> that expresses YFP in response to IAA to our auxin-producing bacteria. These plants were fluorescing more brightly than the controls.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515000" target="_blank"><b>Main Page</b></a> - <b>IaaM - tryptophan-2-mono-oxygenase, BBa_K515000</b>: We have shown that indole 3-acetic acid is being produced in our bacteria. In addition, we have exposed a mutant of <i>Arabidopsis</i> that expresses YFP in response to IAA to our auxin-producing bacteria. These plants were fluorescing more brightly than the controls.</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515001" target="_blank"><b>Main Page</b></a> - <b>Indoleacetamide <del class="diffchange diffchange-inline">hydrolyase</del>, BBa_K515001</b>: We have shown that indole 3-acetic acid is being produced in our bacteria. In addition, we have exposed a mutant of <i>Arabidopsis</i> that expresses YFP in response to IAA to our auxin-producing bacteria. These plants were fluorescing more brightly than the controls.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515001" target="_blank"><b>Main Page</b></a> - <b>Indoleacetamide <ins class="diffchange diffchange-inline">hydrolase</ins>, BBa_K515001</b>: We have shown that indole 3-acetic acid is being produced in our bacteria. In addition, we have exposed a mutant of <i>Arabidopsis</i> that expresses YFP in response to IAA to our auxin-producing bacteria. These plants were fluorescing more brightly than the controls.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515010" target="_blank"><b>Main Page</b></a> - <b>Regulatory pVeg2, BBa_K515010</b>: We discovered that this part is functional and XylE is still expressed.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515010" target="_blank"><b>Main Page</b></a> - <b>Regulatory pVeg2, BBa_K515010</b>: We discovered that this part is functional and XylE is still expressed.</li></div></td></tr>
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</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=167815&oldid=prevRebekkaBauer at 03:06, 22 September 20112011-09-22T03:06:16Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <b>Part:BBa_K112808</b> (Berkeley, iGEM 2008) We have made <del class="diffchange diffchange-inline"><b></del>anti-holin from the Berkeley 2008 lysis cassette available as a <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>stand-alone BioBrick</a></b>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <b>Part:BBa_K112808</b> (Berkeley, iGEM 2008) We have made anti-holin from the Berkeley 2008 lysis cassette available as a <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>stand-alone BioBrick</a></b>.</p></div></td></tr>
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</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=167799&oldid=prevRebekkaBauer at 03:05, 22 September 20112011-09-22T03:05:33Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <b>Part:BBa_K112808</b> We have made <b>anti-holin from the Berkeley 2008 lysis cassette available as a <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>stand-alone BioBrick</a></b>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <b>Part:BBa_K112808</b> <ins class="diffchange diffchange-inline">(Berkeley, iGEM 2008) </ins>We have made <b>anti-holin from the Berkeley 2008 lysis cassette available as a <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>stand-alone BioBrick</a></b>.</p></div></td></tr>
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</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=167791&oldid=prevRebekkaBauer at 03:05, 22 September 20112011-09-22T03:05:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <b>Part:<del class="diffchange diffchange-inline">BBa_K515004</del></b> We have made <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b><del class="diffchange diffchange-inline">anti</del>-<del class="diffchange diffchange-inline">holin</b></del></a> <del class="diffchange diffchange-inline">from the </del><<del class="diffchange diffchange-inline">a href="http:</del>/<del class="diffchange diffchange-inline">/partsregistry.org/Part:BBa_K112808:Experience"><</del>b><del class="diffchange diffchange-inline">Berkeley 2008 lysis cassette</a></b> available as a stand-alone BioBrick</del>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <b>Part:<ins class="diffchange diffchange-inline">BBa_K112808</ins></b> We have made <ins class="diffchange diffchange-inline"><b>anti-holin from the Berkeley 2008 lysis cassette available as a </ins><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b><ins class="diffchange diffchange-inline">stand</ins>-<ins class="diffchange diffchange-inline">alone BioBrick</ins></a></b>.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=167753&oldid=prevRebekkaBauer at 03:03, 22 September 20112011-09-22T03:03:36Z<p></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Parts we have improved</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Parts we have improved</h1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - We have made <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>anti-holin</b></a> from the <a href="http://partsregistry.org/Part:BBa_K112808:Experience"><b>Berkeley 2008 lysis cassette</a></b> available as a stand-alone BioBrick.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - <ins class="diffchange diffchange-inline"><b>Part:BBa_K515004</b> </ins>We have made <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>anti-holin</b></a> from the <a href="http://partsregistry.org/Part:BBa_K112808:Experience"><b>Berkeley 2008 lysis cassette</a></b> available as a stand-alone BioBrick.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ol></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ol></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=167725&oldid=prevRebekkaBauer at 03:02, 22 September 20112011-09-22T03:02:35Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:02, 22 September 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Parts we have improved</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Parts we have improved</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - We have made <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>anti-holin</b></a> from the <a href="http://partsregistry.org/Part:BBa_K112808:Experience"><b>Berkeley 2008 lysis cassette</a></b> available as a stand-alone BioBrick.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ol></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li></ins><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - We have made <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>anti-holin</b></a> from the <a href="http://partsregistry.org/Part:BBa_K112808:Experience"><b>Berkeley 2008 lysis cassette</a></b> available as a stand-alone BioBrick.</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">/ol</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>We've also created and characterised the following parts</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>We've also created and characterised the following parts</h1></div></td></tr>
</table>RebekkaBauerhttp://2011.igem.org/wiki/index.php?title=Team:Imperial_College_London/Data&diff=167708&oldid=prevRebekkaBauer at 03:02, 22 September 20112011-09-22T03:02:02Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:02, 22 September 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/Part:BBa_I13522:Experience" target="_blank"><b>Experience</b></a> - <b>p(TetR) GFP - BBa_I13522</b> (Endy Lab, iGEM 2005): We have added the results of our thermostability assay to the experience page of this part.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://partsregistry.org/Part:BBa_I13522:Experience" target="_blank"><b>Experience</b></a> - <b>p(TetR) GFP - BBa_I13522</b> (Endy Lab, iGEM 2005): We have added the results of our thermostability assay to the experience page of this part.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ol></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ol></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h1>Parts we have improved</h1></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="http://partsregistry.org/Part:BBa_K112808:Experience" target="_blank"><b>Experience</a></b> - We have made <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515004"><b>anti-holin</b></a> from the <a href="http://partsregistry.org/Part:BBa_K112808:Experience"><b>Berkeley 2008 lysis cassette</a></b> available as a stand-alone BioBrick.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>We've also created and characterised the following parts</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>We've also created and characterised the following parts</h1></div></td></tr>
</table>RebekkaBauer