Team:ITESM Mexico/Extras

From 2011.igem.org

(Difference between revisions)
(→Abbreviations and glossary:)
 
(15 intermediate revisions not shown)
Line 1: Line 1:
{{Team:ITESM_Mexico/Top}}
{{Team:ITESM_Mexico/Top}}
-
A compilation of aditional stuff.
+
 
 +
<html><h1 class="myowntitle">Extras</h1> </html>
 +
-----
 +
 
 +
 
 +
::'''Some aditional stuff.'''
 +
 
 +
[[File:Giant_microbe.png|center]]
 +
 
 +
==='''Home made quick saliva DNA extraction protocol:'''===
 +
 
 +
::#Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
 +
::#Prepare a solution with soap and water in a beaker
 +
::#Add the soap solution to the last rinse
 +
::#Add frozen (0°C) etanol  to the last rinse
 +
::#Observe how a DNA thread forms, take it and place it in an eppendorf tube.
 +
 
 +
 
 +
===Abbreviations and glossary:===
 +
 
 +
::*'''Biobrickoatl:'''It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
 +
::*'''Competent cells:''' Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
 +
::*'''CcaS-EnvZ: '''Is the green light receptor that induces the expresión of phycobilisome linker protein.
 +
::*'''CFP:''' Cyan Fluorescent Protein
 +
::*'''Crst:''' Lock for the PbadST (strong, for low concentrations) promoter
 +
::*'''CrWk:''' Lock for the Pbad WK (weak, for high concentration) promoter
 +
::*'''GFP:''' Green Fluorescent Protein
 +
::*'''ho1:''' Heme oxigenase 1
 +
::*'''iTaST:''' Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
 +
::*'''pBADST:''' Strong arabinose promoter for low concentration detection.
 +
::*'''pBADWk:''' Weak arabinose promoter for high concentration detection.
 +
::*'''Pcy4A:''' Phycocyanobilin ferredoxin oxidoreductase.
 +
::*'''RBS:''' Ribosome binding site
 +
::*'''TaST:''' Key for the CrST lock
 +
::*'''TaWk:''' Key for the CrWk lock
 +
::*'''Term:''' Terminator, not the robot (genetic terminator).

Latest revision as of 03:28, 29 September 2011

ITESM MÉXICO

SensE.coli

Igem Itesm

Extras


Some aditional stuff.
Giant microbe.png

Home made quick saliva DNA extraction protocol:

  1. Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
  2. Prepare a solution with soap and water in a beaker
  3. Add the soap solution to the last rinse
  4. Add frozen (0°C) etanol to the last rinse
  5. Observe how a DNA thread forms, take it and place it in an eppendorf tube.


Abbreviations and glossary:

  • Biobrickoatl:It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
  • Competent cells: Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
  • CcaS-EnvZ: Is the green light receptor that induces the expresión of phycobilisome linker protein.
  • CFP: Cyan Fluorescent Protein
  • Crst: Lock for the PbadST (strong, for low concentrations) promoter
  • CrWk: Lock for the Pbad WK (weak, for high concentration) promoter
  • GFP: Green Fluorescent Protein
  • ho1: Heme oxigenase 1
  • iTaST: Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
  • pBADST: Strong arabinose promoter for low concentration detection.
  • pBADWk: Weak arabinose promoter for high concentration detection.
  • Pcy4A: Phycocyanobilin ferredoxin oxidoreductase.
  • RBS: Ribosome binding site
  • TaST: Key for the CrST lock
  • TaWk: Key for the CrWk lock
  • Term: Terminator, not the robot (genetic terminator).
Retrieved from "http://2011.igem.org/Team:ITESM_Mexico/Extras"