Team:HokkaidoU Japan

From 2011.igem.org

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Contents

  • Welcome to the Bio Art Gallery!!

  • Be careful not to contaminate...

  • Meeting, meeting and MEETING!

  • Dr. E. coli get ready for the job

  • Bio Art, please feel free to look around

  • Experiment, experiment and EXPERIMENT!

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Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

BioArt

We held the new type science art gallery "Bio Art" at the center of Sapporo city.

This gallery is not only for our team.You can also enjoy Bio Art on this Web page. You can watch more detail in Human Practice page.

Acknowledgements

Laboratory and Personnel

  • Ken-ichi Yamazaki and Members of Yamazaki Lab
Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
  • Hisatoshi Shida
Molecular Biology, Institute for Genetic Medicine, Hokkaido University

cademic Institutions

  • Salmonella Genetic Stock Center/University of Calgary
Provided E.coli with BAC vector library coding for Type 3 Secretion Apparatus and SlrP signal
  • Graduate School of Environmental Earth Science
  • Department of biological science/School of Sciense
  • Institute for Genetic Medicine