Team:HKUST-Hong Kong/contribution.html

From 2011.igem.org

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<br>Contributions</font></p>
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Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?
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Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</a>
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Is there a local biosafety group, committee, or review board at your institution? What does your local biosafety group think about your project?
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<a href=#q4>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
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<p><b>Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?</b></p>
 
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    <p>iGEM  2011 HKUST team is composed of 29 undergraduate members, 10 advisors, and 3  instructors. Together, we have gone through successes, setbacks, and precious  moments as a team to beget the E. trojan. </p><br>
 +
    <p>The  project, starting from the proposal of its topic to its implementation and  finalization, is carried out independently by the team members. Our instructors  and advisors, however, have  provided us with great technical support and  invaluable experience sharing. Without them, there is no way the project could  reach its current state. Right now, we have submitted eight BioBrick parts, one  of which has been characterized and also a new plasmid vector. All of them were  designed and constructed through a great collaborative effort by our team members. </p><br>
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    <p>Laboratory work apart, our Human Practice Group also held a &quot;Synthetic Biology Workshop&quot; in September and conducted a &ldquo;Synthetic Biology Survey&rdquo; for the general public in Hong Kong (mostly alumni and students of HKUST). The questionnaire was disseminated from August to October this year. The lab tour in the workshop was coordinated by our instructor Dr. Jessica TANG. The survey was originated from IDC and Biofaction in Austria [kindly provided by Markus Schmidt, Lei Pei and Wolfgang Kerbe] and was further modified by our human practice team members. </p>
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    <p>Supports from external parties on data evaluation are documented in details on our <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html">Acknowledgements</a> page.</p>
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    <p>&nbsp;</p>
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    <p>Our  members' detailed work distribution is listed as follows:<br>
 +
      <strong>Project:<em> E. trojan</em></strong></p>
 +
    <p><strong>Project Proposal:</strong> <br>
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      Nathaniel,  Trevor, Theodore</p><br>
 +
    <p><strong>Project Documentation: </strong><br>
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      Trevor, Theodore, Nathaniel, Shirley, Vege, Cynthia, Michael, Kelly, Steven, Claire,  Wendy, Jeff</p><br>
 +
    <p><strong>Lab Work:</strong><br>
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      <strong>1 Strain  Construction</strong><br>
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      Trevor,  Nathaniel, Shirley, Vege, Cynthia Chen, Wayne, Michael, Kelly, Jinny, Bonita, Kwan,  Max, Vincent</p>
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    <p><strong>2 Culture Test</strong><br>
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      Theodore,  Nathaniel, Steven, Claire, Wendy Xu, Yellow, Julia, Peter, Alfie, Susanna, Billy</p>
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    <p><strong>3 Construction and  characterization of BioBricks</strong> <br>
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      Nathaniel,  Trevor, Theodore, Shirley, Vege, Jinny, Kelly, Michael, Bonita, Wendy Xu,  Steven, Claire.</p><br>
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    <p><strong>Human Practice:</strong></p>
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    <p><strong>1 Synthetic Biology  Workshop</strong><br>
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    <br />
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      <strong>Organizers: </strong><br>
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      Wendy  Xu, Theodore, Steven, Claire, Michael, Jeff, Shirley, Max, Kwan, Ting<br>
 +
      <br />
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  <strong>Card Game-The Life  of <em>E.coli </em>Designer: </strong><br>
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      Steven,  Jeff, Theodore, Shirley.<br>
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      <br/>
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  <strong>Video on game rule:</strong><br>
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      Shirley,  Audrey<br>
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  <strong>&ldquo;Be a Plasmid  Engineer&rdquo; Designer:</strong><br>
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      Claire,  Michael<strong> </strong><br>
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<br />
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  <strong>Helpers</strong>: <br>
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      Wendy  Xu,Theodore, Steven, Claire, Michael, Jeff, Shirley, Max, Kwan, Ting, Yimo,  Julie, Joyce, Vege, Wendy Lu.<br>
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<br />
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  <strong>Institutional  facilitation:</strong><br>
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      Prof.  King Lau Chow ( team instructor), Dr. Jessica Ce Mun Tang( team instructor), Ms  Kit Ng( team instructor)</p><br>
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    <p><strong>2 Synthetic Biology  Survey</strong><br>
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      <strong>Modification:</strong><br>
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      Shirley,  Steven, Wendy<br>
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      <br />
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  <strong>Data analysis:</strong><br>
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      Shirley,  Jeff, Steven, Wendy Xu, Claire, Cynthia Chen<br>
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      <br />
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  <strong>Report:</strong><br>
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      Shirley<br>
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      <br />
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  <strong>Original design of  the survey before adaptation: </strong><br>
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      Markus Schmidt, Lei Pei and Wolfgang Kerbe of IDC  and Biofaction, Austria </p><br>
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    <p><strong>Others:</strong>
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    <br />
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      <strong>1 Secretary of  general meeting:</strong> <br>
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      Claire,  Shirley<br>
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      <br />
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  <strong>2 Wiki:</strong><br>
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  <br />
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      a.<strong> </strong><strong>Design and construction of Wiki</strong> <br>
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      Ting,  Jeff, Jack<br>
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      <br />
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      b. <strong>Editing of wiki </strong><br>
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      Ting,  Jeff, Jack, Jinny, Nathaniel, Trevor, Theodore, Steven, Claire, Wendy Xu,  Kelly, Shirley<br>
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      <br />
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  <strong>3 Poster</strong> <br>
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      Vege<br>
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Parts, ideas, and strains of model organisms in the 2011 HKUST iGEM team will not pose any threat to safety of researchers, public or environment. All biological parts, when functioning correctly, should not produce toxins or harmful chemicals to other organisms. Model organisms used include <i>Escherichia coli</i> DH10b, BW25113 and BW25141. They are non-virulent strains and are common in microbiology laboratories for basic biology studies.
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  <strong>4 T-shirt and Mascot</strong><br>
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      Susanna, Vege, Yellow, Alfie, Max<br>
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  <strong>5 Modeling:</strong><br>
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      Cynthia  Wang, Nathaniel</p>
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<p><b>Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</b></p>
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<br>
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The new BioBrick parts that are currently under design encode non-hazardous genes and should be harmless, with only one exception. That BioBrick part might slightly raise the resistance of <i>Escherichia coli</i> to certain antibiotics, but the effect will be limited to the bacteria cultured for testing experiments.  The part encodes a multi-efflux pump that mainly transports aminoglycosides out of the cytosol. Yet we believe it does not have significant impact on the general safety of the environment since, in theory, it would only raise the minimum inhibition concentration (MIC) of the host organism by no more than 10 µg/ml.</p><p>
 
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To avoid any potential hazards, Biological Safety Level 1 requirements are strictly followed. Biological wastes and all equipment that have physical contacts with biological samples are bleached (optional) and autoclaved before disposal or reuse.
 
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top>
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<b><font color="#FFE1E1" size=3>Home</font></b>
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<p><b>Is there a local biosafety group, committee, or review board at your institution? What does your local biosafety group think about your project?
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The Biosafety Committee of HKUST Health, Safety & Environmental Office keeps track of all safety regulations and practices in the campus. They address all issues arisen from current experimental procedures taking place and keeps an eye on ethical practices that involves the handling of genetically modified cells and animals, the latter of which does not apply in this project, since animal study will not be included in the project described here.
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Procedures currently involved in iGEM 2011 HKUST team mainly concern molecular cloning and constructing reporter systems for antibiotics resistance. Constructs and parts will NOT be distributed or released to the environment. Guidelines from NIH recombinant DNA regulations are followed as closely as possible. Therefore details of our project had not, and will not evolve into any public safety issues that need to be addressed.
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<b><font color="green">Our Project</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font>
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<p align=right>Back</p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p>
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<p align="center" valign="baseline">
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<b><font color="green">Experiments and Results</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html"  target=_top>Strain Construction</a><font color="green"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html"  target=_top>Culture Test</a><font color="green"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html"  target=_top>Modeling</a><br></p>
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<p align="center" valign="baseline">
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<b><font color="green">Miscellaneous</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p>
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<p><b>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</b>
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<p>
 
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We propose to include a suicide system inside all BioBrick plasmid backbones. Using toxin-antitoxin systems coupled with the appropriate promoters, we can trigger self-destruction in any cell that has taken up the plasmid using particular inducers or by subjecting undesired hosts to a slightly varied physical environment. This can safeguard against undesired gain of resistance or gain of advantage by the manipulated organism, or contaminant species that have obtained the plasmid through horizontal gene transfer.
 
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<b><font color="#FFF4D0">iGEM Resources</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p>
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<b><font color="#FFF4D0">The Team</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br></p>
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<b><font color="#FFF4D0">Achievements</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements</a><font color="#FFF4D0"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p>
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<b><font color="#FFF4D0">BioBricks</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p>
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Our Project</font></b></p>
 
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<span style="line-height:1; font-weight:600">Experiments and Results</span><br>
 
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<a href="asm.html"  target=_top>Strain construction</a> |
 
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<b><font color="#FFE0E0">Human Practice</font></b></p>
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iGEM Resources</font></b></p>
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<span style="line-height:0.7; font-weight:600">The Team</span><br>
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Human Practice</font></b></p>
 
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Latest revision as of 14:34, 27 October 2011


Contributions



iGEM 2011 HKUST team is composed of 29 undergraduate members, 10 advisors, and 3 instructors. Together, we have gone through successes, setbacks, and precious moments as a team to beget the E. trojan.


The project, starting from the proposal of its topic to its implementation and finalization, is carried out independently by the team members. Our instructors and advisors, however, have provided us with great technical support and invaluable experience sharing. Without them, there is no way the project could reach its current state. Right now, we have submitted eight BioBrick parts, one of which has been characterized and also a new plasmid vector. All of them were designed and constructed through a great collaborative effort by our team members.


Laboratory work apart, our Human Practice Group also held a "Synthetic Biology Workshop" in September and conducted a “Synthetic Biology Survey” for the general public in Hong Kong (mostly alumni and students of HKUST). The questionnaire was disseminated from August to October this year. The lab tour in the workshop was coordinated by our instructor Dr. Jessica TANG. The survey was originated from IDC and Biofaction in Austria [kindly provided by Markus Schmidt, Lei Pei and Wolfgang Kerbe] and was further modified by our human practice team members.

Supports from external parties on data evaluation are documented in details on our Acknowledgements page.

 

Our members' detailed work distribution is listed as follows:
Project: E. trojan

Project Proposal:
Nathaniel, Trevor, Theodore


Project Documentation:
Trevor, Theodore, Nathaniel, Shirley, Vege, Cynthia, Michael, Kelly, Steven, Claire, Wendy, Jeff


Lab Work:
1 Strain Construction
Trevor, Nathaniel, Shirley, Vege, Cynthia Chen, Wayne, Michael, Kelly, Jinny, Bonita, Kwan, Max, Vincent

2 Culture Test
Theodore, Nathaniel, Steven, Claire, Wendy Xu, Yellow, Julia, Peter, Alfie, Susanna, Billy

3 Construction and characterization of BioBricks
Nathaniel, Trevor, Theodore, Shirley, Vege, Jinny, Kelly, Michael, Bonita, Wendy Xu, Steven, Claire.


Human Practice:

1 Synthetic Biology Workshop

Organizers:
Wendy Xu, Theodore, Steven, Claire, Michael, Jeff, Shirley, Max, Kwan, Ting

Card Game-The Life of E.coli Designer:
Steven, Jeff, Theodore, Shirley.

Video on game rule:
Shirley, Audrey

“Be a Plasmid Engineer” Designer:
Claire, Michael

Helpers:
Wendy Xu,Theodore, Steven, Claire, Michael, Jeff, Shirley, Max, Kwan, Ting, Yimo, Julie, Joyce, Vege, Wendy Lu.

Institutional facilitation:
Prof. King Lau Chow ( team instructor), Dr. Jessica Ce Mun Tang( team instructor), Ms Kit Ng( team instructor)


2 Synthetic Biology Survey

Modification:
Shirley, Steven, Wendy

Data analysis:
Shirley, Jeff, Steven, Wendy Xu, Claire, Cynthia Chen

Report:
Shirley

Original design of the survey before adaptation:
Markus Schmidt, Lei Pei and Wolfgang Kerbe of IDC and Biofaction, Austria


Others:
1 Secretary of general meeting:
Claire, Shirley

2 Wiki:

a. Design and construction of Wiki
Ting, Jeff, Jack

b. Editing of wiki
Ting, Jeff, Jack, Jinny, Nathaniel, Trevor, Theodore, Steven, Claire, Wendy Xu, Kelly, Shirley

3 Poster
Vege

4 T-shirt and Mascot
Susanna, Vege, Yellow, Alfie, Max

5 Modeling:
Cynthia Wang, Nathaniel

Home

Our Project

Overview | Data Page

Experiments and Results

Strain Construction | Culture Test | Modeling

Miscellaneous

Notebook

iGEM Resources

Acknowledgements

The Team

iGEM Member List | Contributions

Achievements

Medal Requirements | BioSafety

BioBricks

Master List & Characterization Data

Human Practice

Workshop | Survey

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