Team:HKUST-Hong Kong/characterization.html

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<blockquote>
<blockquote>
1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> –oriR101 & repA101-ts: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.<br><br>
1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> –oriR101 & repA101-ts: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/b/b2/Ust_BBa_K524000.png" width="700px">
 +
<br >
2. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> –pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
2. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> –pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/8/8d/Ust_BBa_K524001%266.png" width="700px">
 +
<br >
3. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> –pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
3. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> –pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/d/d9/Ust_BBa_K524002%267.png" width="700px">
 +
<br >
4. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthatase.<br><br>
4. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthatase.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/2/2a/Ust_BBa_K524003.png" width="700px">
 +
<br >
5. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> –pir gene: the pir gene encodes the autogenously regulated pi protein.<br><br>
5. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> –pir gene: the pir gene encodes the autogenously regulated pi protein.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/e/e3/Ust_BBa_K524004.png" width="700px">
 +
<br >
6. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
6. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/2/2b/Ust_BBa_K524005.png" width="700px">
 +
<br />
 +
7. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> –RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
7. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> –RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/8/8d/Ust_BBa_K524001%266.png" width="700px">
 +
<br >
8. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> –RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
8. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> –RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/d/d9/Ust_BBa_K524002%267.png" width="700px">
 +
<br >
 +
9. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br>
9. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/5/5d/Ust_BBa_K524100.png" width="700px">
 +
<br >
</blockquote>
</blockquote>

Revision as of 15:12, 5 October 2011


BioBricks Master List

&

Characterization Data




1. BBa_K524000 –oriR101 & repA101-ts: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.


2. BBa_K524001 –pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.


3. BBa_K524002 –pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.


4. BBa_K524003 – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthatase.


5. BBa_K524004 –pir gene: the pir gene encodes the autogenously regulated pi protein.


6. BBa_K524005 – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.


7. BBa_K524006 –RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.


8. BBa_K524007 –RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.


9. BBa_K524100 – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.


Characterization Data for BBa_K524000

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