Team:HKUST-Hong Kong/characterization.html

From 2011.igem.org

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1. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>Main Page</font></a> –pLac + RBS + sfGFP1-10, BBa_K524001: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
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2. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>Main Page</font></a> –pLac + RBS + sfGFP11 + double terminator, BBa_K524002: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
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3. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>Main Page</font></a> – nadE gene + double terminator, BBa_K524003: an essential gene that encodes constitutive expressed NAD+ synthatase.<br><br>
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4. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>Main Page</font></a> –pir gene, BBa_K524004: the pir gene encodes the autogenously regulated pi protein.<br><br>
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5. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>Main Page</font></a> – pToolkit, BBa_K524005: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
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6. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>Main Page</font></a> –RBS + sfGFP1-10, BBa_K524006: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
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7. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>Main Page</font></a> –RBS + sfGFP11 + double terminator, BBa_K524007: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
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8. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>Main Page</font></a> – bcr CDS + double terminator B0015, BBa_K524100: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degree of resistance to 5 antibiotics.<br><br>
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Revision as of 10:42, 5 October 2011

Biobricks Master List & Characterization Data



1. Main Page –pLac + RBS + sfGFP1-10, BBa_K524001: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.

2. Main Page –pLac + RBS + sfGFP11 + double terminator, BBa_K524002: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.

3. Main Page – nadE gene + double terminator, BBa_K524003: an essential gene that encodes constitutive expressed NAD+ synthatase.

4. Main Page –pir gene, BBa_K524004: the pir gene encodes the autogenously regulated pi protein.

5. Main Page – pToolkit, BBa_K524005: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.

6. Main Page –RBS + sfGFP1-10, BBa_K524006: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.

7. Main Page –RBS + sfGFP11 + double terminator, BBa_K524007: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.

8. Main Page – bcr CDS + double terminator B0015, BBa_K524100: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degree of resistance to 5 antibiotics.

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