Team:HKUST-Hong Kong
From 2011.igem.org
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<span style="line-height:2; font-weight:600"> | <span style="line-height:2; font-weight:600"> | ||
- | <a href="overview.html" target=_top>Overview</a> | | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a> | |
- | <a href="data.html" target=_top>Data Page</a><br> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br> |
Experiments and Results<br> | Experiments and Results<br> | ||
- | <a href="asm.html" target=_top>Strain Construction</a> | | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html" target=_top>Strain Construction</a> | |
- | <a href="mic.html" target=_top>Culture Tests</a> | | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html" target=_top>Culture Tests</a> | |
- | <a href="modeling.html" target=_top>Modeling</a><br> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html" target=_top>Modeling</a><br> |
Miscellaneous<br> | Miscellaneous<br> | ||
- | <a href="notebook.html" target=_top>Notebook</a> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a> |
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<span style="line-height:2; font-weight:600"> | <span style="line-height:2; font-weight:600"> | ||
- | <a href="acknowledgement.html" target=_top>Acknowledgements</a><br> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a><br> |
+ | The Team<br> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGem Member List</a> | | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br> | ||
+ | BioBricks<br> | ||
Achievements<br> | Achievements<br> | ||
- | <a href="medal.html" target=_top>Medal Requirements</a> | | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements</a> | |
- | <a href="biosafety.html" target=_top>BioSafety</a><br> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br> |
BioBricks<br> | BioBricks<br> | ||
- | <a href="characterization.html" target=_top>Master List & Characterization Data</a> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a> |
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- | <a href="workshop.html" target=_top>Workshop</a> | | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a> | |
- | <a href="survey.html" target=_top>Survey</a> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a> |
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<p align="center" valign="baseline"> | <p align="center" valign="baseline"> | ||
- | <a href="overview.html" target=_top>Overview</a><font color="green"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font> |
- | <a href="data.html" target=_top>Data Page</a><br></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p> |
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- | <a href="asm.html" target=_top>Strain Construction</a><font color="green"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html" target=_top>Strain Construction</a><font color="green"> | </font> |
- | <a href="mic.html" target=_top>Culture Tests</a><font color="green"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html" target=_top>Culture Tests</a><font color="green"> | </font> |
- | <a href="modeling.html" target=_top>Modeling</a><br></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html" target=_top>Modeling</a><br></p> |
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- | <a href="notebook.html" target=_top>Notebook</a></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p> |
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- | <a href="acknowledgement.html" target=_top>Acknowledgements</a></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p> |
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- | <a href="team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font> |
- | <a href="contribution.html" target=_top>Contributions</a><br></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br></p> |
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- | <a href="medal.html" target=_top>Medal Requirements<font color="#FFF4D0"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements<font color="#FFF4D0"> | </font> |
- | <a href="biosafety.html" target=_top>BioSafety</a><br></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p> |
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- | <a href="characterization.html" target=_top>Master List & Characterization Data</a><br></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p> |
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- | <a href="workshop.html" target=_top>Workshop<font color="white"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop<font color="white"> | </font> |
- | <a href="survey.html" target=_top>Survey</a><br></p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p> |
Revision as of 11:14, 26 October 2011
Greeting from the 2011 HKUST iGEM Team!
This is the 4th time HKUST has participated in this international synthetic biology competition. |
Overview |
Data Page |
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Acknowledgements |
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Project Abstract It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings suggest that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling. Our team aims to interfere with this signalling through introducing a disruptor E. coli into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling. Along the way, we will also create a new strain of E. coli that utilizes an essential gene (nadE) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol. |
Our Project Experiments and Results
Strain Construction |
Culture Tests |
Modeling Miscellaneous |
iGEM Resources The Team
iGEM Member List |
Contributions Achievements
Medal Requirements |
BioSafety BioBricks |
Human Practice |