Team:Glasgow/iGEM Diary

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Video Logs

Want to know how life in the lab is going? Check out our weekly videos!

Week 1 Summary

This week the video is presented by Michal Przydacz. Click here to find out more about Michal!



     This week:
      * Our new lab was set up
      * We created glycerol stocks of E.coli and Pseudomonas strains for future use
      * We had a meeting with Dr Daniel Walker who gave us some really useful information
      * We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them






Week 2 Summary

This week the video is presented by Ellin Hillert. Click here to find out more about Ellin!



 
    This week:
      * We began the week with yet another transformation failure, resulting in our first successful transformants!
      * Our secret strain of E.coli arrived!
      * We each gave a presentation on different aspects of our project









Week 3 Summary

This week the video is presented by Andrew Landels. Click here to find out more about Andrew!



 
    This week:
      * We now have successful transformations of all our biobricks!
      * Our secret strain of E.coli forms really nice biofilms!
      * We had our very first Wiki Whaaaaaam!










Week 4 Summary

This week the video is presented by Pietro Ridone. Click here to find out more about Pietro!



 
    This week:
      * Work to gather data on biofilm growth and dispersal continues.
      * Primers for biobricks arrived
      * The guys have given us a sneak preview of the Norwich presentation
      * We have acquired our own Flouresence Microscope...








Week 5 Summary

This week the video is presented by Chris Wood. Click here to find out more about Chris!



 
    This week:
      * The guys have returned from Norwich with some great insights
      * Everyone is excited to incorporate human practices into the project in a new way
      * We now have a plan for Modelling









Week 6 Summary

This week the video is presented by Hannah Ralph. Click here to find out more about Hannah!



 
    This week:
      * We started ligating our constructs
      * PCR was carried out on our novel biobricks
      * Our secret E.coli strain was successfully transformed with RFP!










Week 7 Summary

This week the video is presented by Emma Campbell. Click here to find out more about Emma!



 
    This week:
      * Edinburgh sent us some biobricks to work with
      * We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis
      * Our poster for the Open Day is looking great!
      * Amsterdam is booked - everyone is really excited!









Week 8 Summary

This week the video is presented by Stewart O'Neill. Click here to find out more about Stewart!



 
    This week:
      * Work on our light responsive constructs and Edinburgh biobricks is continuing
      * An EnvZ negative strain of E.coli arrived from the Registry
      * Modelling is going really well










Week 9 Summary

This week the video is presented by Scott Wood. Click here to find out more about Scott!



 
    This week:
      * PCR and Site Directed Mutagenesis is continuing on our novel biobricks
      * Our work with the EnvZ negative cells is ongoing
      * Modelling looks great - we now have some really great graphs for our dispersal biobricks!