Team:Glasgow/iGEM Diary

From 2011.igem.org

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{{Team:Glasgow/Header}}
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!align="center"|[[Team:Glasgow|Home]]
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<html>
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!align="center"|[[Team:Glasgow/Team|Team]]
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<h1>Video Logs</h1>
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!align="center"|[[Team:Glasgow/iGEM_Diary|iGEM Diary]]
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<p>Want to know how life in the lab is going? Check out our weekly videos!</p>
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Glasgow Official Team Profile]
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!align="center"|[[Team:Glasgow/Project|Project]]
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!align="center"|[[Team:Glasgow/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Glasgow/Modeling|Modeling]]
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!align="center"|[[Team:Glasgow/Gallery|Gallery]]
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!align="center"|[[Team:Glasgow/Safety|Safety]]
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!align="center"|[[Team:Glasgow/Attributions|Attributions]]
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What better way to get to know the team than to have access to daily updates of our conquests and defeats? Using our calendar, click on the date to view our daily activities. Project began on Monday 27th June.
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<h2>Week 1 Summary</h2>
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This week the video is presented by Michal Przydacz. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Michal_Przydacz>here</a> to find out more about Michal!<br/><br/>
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[[image:rose.jpg|thumb|left|300px]]
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<iframe width="560" height="315" src="http://www.youtube.com/embed/0sEjIqsmJxY" frameborder="2" allowfullscreen align=left></iframe>
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<br/><br/><ul>&nbsp;<u>This week:</u><br/>
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<ul>* Our new lab was set up</ul>
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<ul>* We created glycerol stocks of <i>E.coli</i> and <i>Pseudomonas</i> strains for future use</ul>
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<ul>* We had a meeting with <a href=http://www.gla.ac.uk:443/ibls/staff/staff.php?who=Ped|dQ>Dr Daniel Walker</a> who gave us some really useful information</ul>
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<ul>* We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them</ul> </ul>
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<br/><br/><br/><br/><br/><br/>
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<h2>Week 2 Summary</h2>
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This week the video is presented by Ellin Hillert. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Ellin_Hillert>here</a> to find out more about Ellin!<br/><br/>
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<iframe width="560" height="315" src="http://www.youtube.com/embed/7wHpCv_hCOY" frameborder="2" allowfullscreen align=LEFT></iframe>
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!align="center"|{{#calendar: title=Glasgow |year=2011 | month=06}}
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<br/><br/>&nbsp;<ul><u>This week:</u><br/>
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!align="center"|{{#calendar: title=Glasgow |year=2011 | month=07}}
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<ul>* We began the week with yet another transformation failure, resulting in our first successful transformants!</ul>
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<ul>* Our secret strain of <i>E.coli</i> arrived!</ul>
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!align="center"|{{#calendar: title=Glasgow |year=2011 | month=08}}
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<ul>* We each gave a presentation on different aspects of our project</ul>
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!align="center"|{{#calendar: title=Glasgow |year=2011 | month=09}}
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</ul>
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<br/>
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<br/><br/><br/><br/><br/><br/><br/><br/>
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<h2>Week 3 Summary</h2>
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This week the video is presented by Andrew Landels. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Andrew_Landels>here</a> to find out more about Andrew!<br /><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/MhnMF-s4DRo" frameborder="2" allowfullscreen align=LEFT></iframe>
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<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* We now have successful transformations of all our biobricks!</ul>
 +
<ul>* Our secret strain of <i>E.coli</i> forms really nice biofilms!</ul>
 +
<ul>* We had our very first Wiki Whaaaaaam!</ul>
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</ul>
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<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
 
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<h2>Week 4 Summary</h2>
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This week the video is presented by Pietro Ridone.  Click <a href=https://2011.igem.org/Team:Glasgow/Team/Pietro_Ridone>here</a> to find out more about Pietro!<br/><br/>
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<iframe width="560" height="315" src="http://www.youtube.com/embed/BpCmEpKz-h4" frameborder="2" allowfullscreen align=LEFT></iframe></center>
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<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* Work to gather data on biofilm growth and dispersal continues.</ul>
 +
<ul>* Primers for biobricks arrived</ul>
 +
<ul>* The guys have given us a sneak preview of the Norwich presentation</ul>
 +
<ul>* We have acquired our own Flouresence Microscope...</ul>
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</ul>
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<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
 
 +
<h2>Week 5 Summary</h2>
 +
This week the video is presented by Chris Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Chris_Wood>here</a> to find out more about Chris!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/rs6vdCWG8yU" frameborder="2" allowfullscreen align=left></iframe></center>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* The guys have returned from Norwich with some great insights</ul>
 +
<ul>* Everyone is excited to incorporate human practices into the project in a new way</ul>
 +
<ul>* We now have a plan for Modelling</ul>
 +
</ul>
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<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
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<h2>Week 6 Summary</h2>
 +
This week the video is presented by Hannah Ralph. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Hannah_Ralph>here</a> to find out more about Hannah!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/6S6KtfYjfg8" frameborder="2" allowfullscreen align=left></iframe>
 +
 
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* We started ligating our constructs</ul>
 +
<ul>* PCR was carried out on our novel biobricks</ul>
 +
<ul>* Our secret <i>E.coli</i> strain was successfully transformed with RFP!</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
 
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<h2>Week 7 Summary</h2>
 +
This week the video is presented by Emma Campbell. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Emma_Campbell>here</a> to find out more about Emma!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/epBvEuJJtds" frameborder="2" allowfullscreen align=left></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* Edinburgh sent us some biobricks to work with</ul>
 +
<ul>* We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis</ul>
 +
<ul>* Our poster for the Open Day is looking great!</ul>
 +
<ul>* Amsterdam is booked - everyone is really excited!</ul>
 +
</ul>
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<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
<h2>Week 8 Summary</h2>
 +
This week the video is presented by Stewart O'Neill. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Stewart_O%27Neill>here</a/> to find out more about Stewart!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/ZiJpsuw36nM" frameborder="2" allowfullscreen align=left></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* Work on our light responsive constructs and Edinburgh biobricks is continuing</ul>
 +
<ul>* An EnvZ negative strain of <i>E.coli</i> arrived from the Registry</ul>
 +
<ul>* Modelling is going really well</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
<h2>Week 9 Summary</h2>
 +
This week the video is presented by Scott Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Scott_Wood>here</a> to find out more about Scott!<br/><br>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/ePeOcq4zEKY" frameborder="2" allowfullscreen align=left></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* PCR and Site Directed Mutagenesis is continuing on our novel biobricks</ul>
 +
<ul>* Our work with the EnvZ negative cells is ongoing</ul>
 +
<ul>* Modelling looks great - we now have some really great graphs for our dispersal biobricks!</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
</html>

Latest revision as of 22:59, 20 September 2011

Video Logs

Want to know how life in the lab is going? Check out our weekly videos!

Week 1 Summary

This week the video is presented by Michal Przydacz. Click here to find out more about Michal!



     This week:
      * Our new lab was set up
      * We created glycerol stocks of E.coli and Pseudomonas strains for future use
      * We had a meeting with Dr Daniel Walker who gave us some really useful information
      * We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them






Week 2 Summary

This week the video is presented by Ellin Hillert. Click here to find out more about Ellin!



 
    This week:
      * We began the week with yet another transformation failure, resulting in our first successful transformants!
      * Our secret strain of E.coli arrived!
      * We each gave a presentation on different aspects of our project









Week 3 Summary

This week the video is presented by Andrew Landels. Click here to find out more about Andrew!



 
    This week:
      * We now have successful transformations of all our biobricks!
      * Our secret strain of E.coli forms really nice biofilms!
      * We had our very first Wiki Whaaaaaam!










Week 4 Summary

This week the video is presented by Pietro Ridone. Click here to find out more about Pietro!



 
    This week:
      * Work to gather data on biofilm growth and dispersal continues.
      * Primers for biobricks arrived
      * The guys have given us a sneak preview of the Norwich presentation
      * We have acquired our own Flouresence Microscope...








Week 5 Summary

This week the video is presented by Chris Wood. Click here to find out more about Chris!



 
    This week:
      * The guys have returned from Norwich with some great insights
      * Everyone is excited to incorporate human practices into the project in a new way
      * We now have a plan for Modelling









Week 6 Summary

This week the video is presented by Hannah Ralph. Click here to find out more about Hannah!



 
    This week:
      * We started ligating our constructs
      * PCR was carried out on our novel biobricks
      * Our secret E.coli strain was successfully transformed with RFP!










Week 7 Summary

This week the video is presented by Emma Campbell. Click here to find out more about Emma!



 
    This week:
      * Edinburgh sent us some biobricks to work with
      * We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis
      * Our poster for the Open Day is looking great!
      * Amsterdam is booked - everyone is really excited!









Week 8 Summary

This week the video is presented by Stewart O'Neill. Click here to find out more about Stewart!



 
    This week:
      * Work on our light responsive constructs and Edinburgh biobricks is continuing
      * An EnvZ negative strain of E.coli arrived from the Registry
      * Modelling is going really well










Week 9 Summary

This week the video is presented by Scott Wood. Click here to find out more about Scott!



 
    This week:
      * PCR and Site Directed Mutagenesis is continuing on our novel biobricks
      * Our work with the EnvZ negative cells is ongoing
      * Modelling looks great - we now have some really great graphs for our dispersal biobricks!









Retrieved from "http://2011.igem.org/Team:Glasgow/iGEM_Diary"