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From 2011.igem.org

Contents 1 Purifictaion 2 green light receptor 2.1 Ligation of Ccas in pSB1C3 2.2 Transformation of PCR product Ccas 3 blue light receptor 3.1 Transformation of Lov-Tap 4 Gel of digested parts and ligation 4.1 Gel of digested parts 4.2 Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.

Purifictaion

Investigators: Julia

Purification of digested PCR products with the PCR purification-Kit.

green light receptor

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of PCR product Ccas

Investigators: Jakob

Comments: Colonies had to be picked.

blue light receptor

Transformation of Lov-Tap

Investigators: Jakob

• Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
• Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
• Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)

Gel of digested parts and ligation

Investigators: Rügiger

Gel of digested parts

• Vector: pSB1K3
• GFP-plastic-binding-domain
• Promotor-RBS (PR1-PR6)

Comments: GFP was excised. Other inserts were not stained properly.

Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.