Team:Freiburg/Notebook/5 July
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24. Labday
Purifictaion
Investigators: Julia
Purification of digested PCR products with the PCR purification-Kit.
Ligation of Ccas in pSB1C3
Investigators: Julia
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.
Transformation of Lov-Tap
Investigators: Jakob
- Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
- Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
- Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)
Transformation of PCR product Ccas
Investigators: Jakob
Colonies had to be picked.