Team:Freiburg/Notebook/5 July

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Contents

24. Labday


Purifictaion

Investigators: Julia

Purification of digested PCR products with the PCR purification-Kit.

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of Lov-Tap

Investigators: Jakob

  • Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
  • Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
  • Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)

Transformation of PCR product Ccas

Investigators: Jakob

Transformation of PCR product Ccas