Team:Freiburg/Notebook/5 July

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==Purification==
 
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'''Investigators: Julia'''
 
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Purification of digested PCR products with the PCR purification-Kit.
 
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
===Ligation of Ccas in pSB1C3===
===Ligation of Ccas in pSB1C3===

Revision as of 11:07, 2 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of PCR product Ccas

Investigators: Jakob

Comments: Colonies had to be picked.

blue light receptor

Transformation of Lov-Tap

Investigators: Jakob

  • Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
  • Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
  • Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)



Precipitator

Gel of digested parts and ligation

Investigators: Rügiger

Gel of digested parts:

  • Vector: pSB1K3
  • GFP-plastic-binding-domain
  • Promotor-RBS (PR1-PR6)


Comments: GFP was excised. Other inserts were not stained properly.

Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.

                       

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