"
Team:Freiburg/Notebook/5 July
From 2011.igem.org
(Difference between revisions)
(→Gel of digested parts and ligation) |
(→Gel of digested parts) |
||
| Line 38: | Line 38: | ||
'''Investigators: Rügiger''' | '''Investigators: Rügiger''' | ||
| - | + | Gel of digested parts: | |
*Vector: pSB1K3 | *Vector: pSB1K3 | ||
*GFP-plastic-binding-domain | *GFP-plastic-binding-domain | ||
| Line 46: | Line 46: | ||
<span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | <span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | ||
| - | |||
===Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.=== | ===Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.=== | ||
Revision as of 10:22, 15 July 2011
Contents |
Purifictaion
Investigators: Julia
Purification of digested PCR products with the PCR purification-Kit.
green light receptor
Ligation of Ccas in pSB1C3
Investigators: Julia
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.
Transformation of PCR product Ccas
Investigators: Jakob
Comments: Colonies had to be picked.
blue light receptor
Transformation of Lov-Tap
Investigators: Jakob
- Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
- Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
- Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)
Precipitator
Gel of digested parts and ligation
Investigators: Rügiger
Gel of digested parts:
- Vector: pSB1K3
- GFP-plastic-binding-domain
- Promotor-RBS (PR1-PR6)
Comments: GFP was excised. Other inserts were not stained properly.
