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Team:Freiburg/Notebook/5 July
From 2011.igem.org
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'''Investigators: Rügiger''' | '''Investigators: Rügiger''' | ||
Revision as of 10:22, 15 July 2011
Contents |
Purifictaion
Investigators: Julia
Purification of digested PCR products with the PCR purification-Kit.
green light receptor
Ligation of Ccas in pSB1C3
Investigators: Julia
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.
Transformation of PCR product Ccas
Investigators: Jakob
Comments: Colonies had to be picked.
blue light receptor
Transformation of Lov-Tap
Investigators: Jakob
- Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
- Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
- Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)
Precipitator
Gel of digested parts and ligation
Investigators: Rügiger
Gel of digested parts
- Vector: pSB1K3
- GFP-plastic-binding-domain
- Promotor-RBS (PR1-PR6)
Comments: GFP was excised. Other inserts were not stained properly.
