"
Team:Freiburg/Notebook/5 July
From 2011.igem.org
(Difference between revisions)
(→Transformation of PCR product Ccas) |
(→Gel of digested parts and ligation) |
||
| Line 33: | Line 33: | ||
'''Investigators: Rügiger''' | '''Investigators: Rügiger''' | ||
| - | + | Gel of digested parts: | |
*Vector: pSB1K3 | *Vector: pSB1K3 | ||
*GFP-plastic-binding-domain | *GFP-plastic-binding-domain | ||
| Line 41: | Line 41: | ||
<span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | <span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | ||
| + | |||
| + | |||
| + | Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3. | ||
Revision as of 10:26, 14 July 2011
Contents |
24. Labday
Purifictaion
Investigators: Julia
Purification of digested PCR products with the PCR purification-Kit.
Ligation of Ccas in pSB1C3
Investigators: Julia
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.
Transformation of Lov-Tap
Investigators: Jakob
- Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
- Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
- Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)
Transformation of PCR product Ccas
Investigators: Jakob
Comments: Colonies had to be picked.
Gel of digested parts and ligation
Investigators: Rügiger
Gel of digested parts:
- Vector: pSB1K3
- GFP-plastic-binding-domain
- Promotor-RBS (PR1-PR6)
Comments: GFP was excised. Other inserts were not stained properly.
Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.
