Team:Freiburg/Notebook/5 July

From 2011.igem.org

(Difference between revisions)
(Transformation of Lov-Tap)
(Ligation of Ccas in pSB1C3)
Line 14: Line 14:
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.
-
 
-
<br/>
 
==<span style="color:lightblue;">Transformation of Lov-Tap</span>==
==<span style="color:lightblue;">Transformation of Lov-Tap</span>==

Revision as of 10:18, 14 July 2011

Contents

24. Labday


Purifictaion

Investigators: Julia

Purification of digested PCR products with the PCR purification-Kit.

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of Lov-Tap

Investigators: Jakob

  • Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
  • Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
  • Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)

Transformation of PCR product Ccas

Investigators: Jakob

Transformation of PCR product Ccas