Team:Freiburg/Notebook/5 July
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+ | <div id="notebook-page-header"> | ||
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+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/4_July">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 5 July </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/7_July">Next entry</a> | ||
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+ | ==<span style="color:green;">green light receptor</span>== | ||
+ | ==Purification== | ||
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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===Ligation of Ccas in pSB1C3=== | ===Ligation of Ccas in pSB1C3=== | ||
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Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector. | Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector. | ||
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===Transformation of PCR product Ccas=== | ===Transformation of PCR product Ccas=== | ||
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<span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | <span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | ||
- | + | Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3. | |
+ | {{:Team:Freiburg/Templates/footer}} |
Latest revision as of 00:52, 22 September 2011