Team:Freiburg/Notebook/5 July

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==Purifictaion==
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/4_July">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 5 July </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/7_July">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
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==Purification==
'''Investigators: Julia'''
'''Investigators: Julia'''
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===Ligation of Ccas in pSB1C3===
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==<span style="color:green;">Ligation of Ccas in pSB1C3</span>==
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'''Investigators: Julia'''
'''Investigators: Julia'''
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===Transformation of PCR product Ccas===
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==<span style="color:darkblue;">Transformation of Lov-Tap</span>==
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'''Investigators: Jakob'''
'''Investigators: Jakob'''
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*Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
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<span style="color:blue;">Comments: Colonies had to be picked.</span>
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*Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
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*Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)
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==<span style="color:green;">Transformation of PCR product Ccas</span>==
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==<span style="color:darkblue;">blue light receptor</span>==
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===Transformation of Lov-Tap===
'''Investigators: Jakob'''
'''Investigators: Jakob'''
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<span style="color:blue;">Comments: Colonies had to be picked.</span>
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*Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
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*Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
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*Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)
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==<span style="color:grey;">Gel of digested parts and ligation</span>==
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==<span style="color:grey;">Precipitator</span>==
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===Gel of digested parts and ligation===
'''Investigators: Rügiger'''
'''Investigators: Rügiger'''
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===Gel of digested parts===
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Gel of digested parts:
*Vector: pSB1K3
*Vector: pSB1K3
*GFP-plastic-binding-domain
*GFP-plastic-binding-domain
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<span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span>
<span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span>
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Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.
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===Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.===
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{{:Team:Freiburg/Templates/footer}}

Latest revision as of 00:52, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Purification

Investigators: Julia

Purification of digested PCR products with the PCR purification-Kit.

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of PCR product Ccas

Investigators: Jakob

Comments: Colonies had to be picked.

blue light receptor

Transformation of Lov-Tap

Investigators: Jakob

  • Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
  • Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
  • Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)



Precipitator

Gel of digested parts and ligation

Investigators: Rügiger

Gel of digested parts:

  • Vector: pSB1K3
  • GFP-plastic-binding-domain
  • Promotor-RBS (PR1-PR6)


Comments: GFP was excised. Other inserts were not stained properly.

Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.

                       

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