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Team:Freiburg/Notebook/5 July
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| - | + | {{:Team:Freiburg/Templates/header}} | |
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| - | == | + | <html> |
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/4_July">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 5 July </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/7_July">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
| + | ==<span style="color:green;">green light receptor</span>== | ||
| + | ==Purification== | ||
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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Purification of digested PCR products with the PCR purification-Kit. | Purification of digested PCR products with the PCR purification-Kit. | ||
<br/> | <br/> | ||
| - | + | <br/> | |
| - | == | + | ===Ligation of Ccas in pSB1C3=== |
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector. | Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector. | ||
| + | <br/> | ||
| + | <br/> | ||
| + | ===Transformation of PCR product Ccas=== | ||
| + | '''Investigators: Jakob''' | ||
| + | |||
| + | <span style="color:blue;">Comments: Colonies had to be picked.</span> | ||
| + | <br/> | ||
<br/> | <br/> | ||
| - | ==<span style="color: | + | ==<span style="color:darkblue;">blue light receptor</span>== |
| + | ===Transformation of Lov-Tap=== | ||
'''Investigators: Jakob''' | '''Investigators: Jakob''' | ||
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*Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3) | *Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3) | ||
*Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3) | *Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3) | ||
| + | <br/> | ||
| + | <br/> | ||
| + | ==<span style="color:grey;">Precipitator</span>== | ||
| + | ===Gel of digested parts and ligation=== | ||
| + | '''Investigators: Rügiger''' | ||
| - | + | Gel of digested parts: | |
| + | *Vector: pSB1K3 | ||
| + | *GFP-plastic-binding-domain | ||
| + | *Promotor-RBS (PR1-PR6) | ||
| - | |||
| - | <span style="color:blue;"> | + | |
| + | <span style="color:blue;">Comments: GFP was excised. Other inserts were not stained properly. </span> | ||
| + | |||
| + | Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3. | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 00:52, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
