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Team:Freiburg/Notebook/13 July
From 2011.igem.org
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<span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span> | <span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span> | ||
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| + | Miniprep of the plastik-binding domain: | ||
| + | |||
| + | {| cellpadding="10" cellspacing="0" border="1" | ||
| + | |samples | ||
| + | |DNA concentration | ||
| + | |280/230 | ||
| + | |260/230 | ||
| + | |- | ||
| + | |M2a alpha/M2a* alpha | ||
| + | |66.5/91.6 | ||
| + | |1.84/1.75 | ||
| + | |1.76/1.40 | ||
| + | |- | ||
| + | |M2a beta/M2a* beta | ||
| + | |} | ||
Revision as of 12:30, 15 July 2011
Contents |
red light receptor
Investigators: Julia
Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.
Miniprep
Investigators: Jakob
Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:
| samples | DNA concentration | 260/280 | 230 |
| cph8a | 33.7 | 1.79 | 1.74 |
| cph8b | 41.2 | 1.79 | 1.39 |
| cph8c | 24.1 | 1.87 | 1.47 |
| cph8d | 30 | 1.74 | 1.84 |
| cph8e | 34 | 1.81 | 1.86 |
| cph8f | 30 | 1.83 | 1.81 |
Testdigest
Investigators: Julia
Testdigest of cph8
- Enzyme 1: EcoRI
- Enzyme 2: PstI
Precipitator
Miniprep
Investigators: Sophie, Sandra
Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.
Miniprep of the plastik-binding domain:
| samples | DNA concentration | 280/230 | 260/230 |
| M2a alpha/M2a* alpha | 66.5/91.6 | 1.84/1.75 | 1.76/1.40 |
| M2a beta/M2a* beta |
