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Revision as of 12:20, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→Miniprep) ← Older edit		Revision as of 12:26, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→Testdigest) Newer edit →
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	*Enzyme 1: EcoRI		*Enzyme 1: EcoRI
	*Enzyme 2: PstI		*Enzyme 2: PstI
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		+	==<span style="color:grey;">Precipitator</span>==
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		+	===Miniprep===
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		+	'''Investigators: Sophie, Sandra'''
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		+	<span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span>

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Revision as of 12:26, 15 July 2011

Contents 1 red light receptor 1.1 Miniprep 1.2 Testdigest 2 Precipitator 2.1 Miniprep

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:

samples	DNA concentration	260/280	230
cph8a	33.7	1.79	1.74
cph8b	41.2	1.79	1.39
cph8c	24.1	1.87	1.47
cph8d	30	1.74	1.84
cph8e	34	1.81	1.86
cph8f	30	1.83	1.81

Testdigest

Investigators: Julia

Testdigest of cph8

• Enzyme 1: EcoRI
• Enzyme 2: PstI

Precipitator

Miniprep

Investigators: Sophie, Sandra

Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.