Team:Freiburg/Notebook/12 June

From 2011.igem.org

(Difference between revisions)
(→picking clones)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
-
=='''??.Labday'''==
+
{{:Team:Freiburg/Templates/header}}
 +
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/11_June">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 12 June </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/13_June">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
 +
 
 +
==Picking clones==
-
==picking clones==
 
'''Investigators: Theo, Sophie'''
'''Investigators: Theo, Sophie'''
Line 9: Line 23:
Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells.
Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells.
Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15)
Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15)
 +
 +
 +
==<span style="color:red;">red light receptor</span>==
 +
===Amplification of the red-light sensor===
 +
 +
'''Investigators: Theo, Sophie'''
 +
 +
amplified the parts for the red light sensor I15008 and I15009 in LB with 1ul/ml Kanamycin , Overnight at 37C 140RPM<br/>
 +
(Start: 18.30, End: 12:15)
 +
for the main receptor ( BBa_I15010) we have no DNA.
 +
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:19, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Picking clones

Investigators: Theo, Sophie

Comments: On plates PR4, PR6, PR8 and PR 9 cells did not grow. More competent cells were plated out again. Succesful growing on plates PR1, PR2, PR3, PR5 and PR7

Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15)


red light receptor

Amplification of the red-light sensor

Investigators: Theo, Sophie

amplified the parts for the red light sensor I15008 and I15009 in LB with 1ul/ml Kanamycin , Overnight at 37C 140RPM
(Start: 18.30, End: 12:15) for the main receptor ( BBa_I15010) we have no DNA.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/12_June"