Team:EPF-Lausanne/Tools/Gibson assembly

From 2011.igem.org

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(Disadvantages)
(Disadvantages)
 
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* Primers are more complicated to design and longer - they are more expensive and take longer to be produced
* Primers are more complicated to design and longer - they are more expensive and take longer to be produced
* The reaction mastermix is expensive - due to large amounts of Taq ligase needed
* The reaction mastermix is expensive - due to large amounts of Taq ligase needed
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* A lot of negative resluts, du to the tendency of the backbones to close up themselves
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* A lot of negative results, due to the tendency of the plasmid backbone to close up on itself

Latest revision as of 21:41, 18 September 2011