• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

In-Vitro Characterization

In order to generate new libraries of transcription factors, we first need a convenient way to induce mutations at specific positions, to mutate the amino acids involved in DNA binding. Then, once having generated mutants, it is needed to have a precise and high-throughput characterization method - these two requirements being present in the microfluidics MITOMI device that we used.

Characterizing a lot of different mutants will allow to get a better understanding on the the factors influencing the specificity and affinity of a transcription factor. Once with these parameters in hand, we will be able to fine-tune the characteristics of the newly produced TFs.

The new transcription factors mutants characterized in vitro then need to be tested in vitro. This last step is explained in the " in vivo characterization section".