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Team:EPF-Lausanne/Our Project/TetR mutants
From 2011.igem.org
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{{:Team:EPF-Lausanne/Templates/TetRtemplate|title=''In-Vitro'' Characterization}} | {{:Team:EPF-Lausanne/Templates/TetRtemplate|title=''In-Vitro'' Characterization}} | ||
| - | + | When we generate new libraries of transcription factors using site-directed mutagenesis, we need to have a precise and high-throughput characterization method. These two requirements are present in the microfluidics MITOMI device that we used. | |
| - | Characterizing a lot of different mutants will allow to get a better understanding on the the | + | Characterizing a lot of different mutants will allow to get a better understanding on the the mutations influencing the specificity and affinity of a transcription factor. Once with these parameters in hand, we will be able to fine-tune the characteristics of the newly produced TFs. |
Revision as of 02:18, 22 September 2011
In-Vitro Characterization
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhen we generate new libraries of transcription factors using site-directed mutagenesis, we need to have a precise and high-throughput characterization method. These two requirements are present in the microfluidics MITOMI device that we used.
Characterizing a lot of different mutants will allow to get a better understanding on the the mutations influencing the specificity and affinity of a transcription factor. Once with these parameters in hand, we will be able to fine-tune the characteristics of the newly produced TFs.
The new transcription factors mutants characterized in vitro then need to be tested in vitro. This last step is explained in the " in vivo characterization section".
