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Team:EPF-Lausanne/Our Project/TetR mutants
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| + | In order to generate new libraries of transcription factors, we first need a convenient way to induce mutations at specific positions, to mutate the amino acids involved in DNA binding. Then, once having generated mutants, it is needed to have a precise and high-throughput characterization method - these two requirements being present in the microfluidics MITOMI device that we used. | ||
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| + | Characterizing a lot of different mutants will allow to get a better understanding on the the factors influencing the specificity and affinity of a transcription factor. Once with these parameters in hand, we will be able to fine-tune the characteristics of the newly produced TFs. | ||
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| + | The new transcription factors mutants characterized in vitro then need to be tested in vitro. This last step is explained in the " ''in vivo'' characterization section". | ||
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Revision as of 02:15, 22 September 2011
In-Vitro Characterization
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineIn order to generate new libraries of transcription factors, we first need a convenient way to induce mutations at specific positions, to mutate the amino acids involved in DNA binding. Then, once having generated mutants, it is needed to have a precise and high-throughput characterization method - these two requirements being present in the microfluidics MITOMI device that we used.
Characterizing a lot of different mutants will allow to get a better understanding on the the factors influencing the specificity and affinity of a transcription factor. Once with these parameters in hand, we will be able to fine-tune the characteristics of the newly produced TFs.
The new transcription factors mutants characterized in vitro then need to be tested in vitro. This last step is explained in the " in vivo characterization section".
