Team:EPF-Lausanne

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<h2><a href="/Team:EPF-Lausanne/Our_Project/TetR_mutants"><i>In vitro</i> TF characterization</a></h2>
<h2><a href="/Team:EPF-Lausanne/Our_Project/TetR_mutants"><i>In vitro</i> TF characterization</a></h2>
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<p>We used a microfluidic based approach for characterizing TF mutants in vitro. The <a href="/Team:EPF-Lausanne/Tools/MITOMI">MITOMI</a> method allows us to measure absolute binding affinities and specificities of transcription factors. We determined the precise binding energy landscape of the wild type TetR transcription factor. We also generated several TetR transcription factor mutants and determined the specificities of a number of the new variants.</p>
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<p>We used a microfluidic based approach for characterizing TF mutants in vitro. The <a href="/Team:EPF-Lausanne/Tools/MITOMI">MITOMI</a> method allows us to measure absolute binding affinities and specificities of transcription factors. We determined the precise <a href="/Team:EPF-Lausanne/Our_Project/TetR_mutants/MITOMI_data">binding energy landscape</a> of the wild type TetR transcription factor. We also generated <a href="/Team:EPF-Lausanne/Our_Project/TetR_mutants/muTetRs">several TetR transcription factor mutants</a> and determined the specificities of a number of the new variants.</p>
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Revision as of 03:05, 22 September 2011