"

From 2011.igem.org

<link rel="stylesheet" href="https://2011.igem.org/Team:DTU-Denmark/stylesheet?action=raw&ctype=text/css" type="text/css" />

Project description

Non-coding RNA is an active area of research with untapped possibilities for application in biotechnology. In prokaryotes a growing number of small RNA(sRNA) have revealed unsuspected layers of regulation.

We at the DTU-Denmark team are designing a sRNA based regulatory system compatible with the BioBrick standard. Our core ideas are inspired by a novel mechanism of sRNA mediated post-transcriptional regulation. The natural occurring system may provide design solutions enabling tight regulatory control and specificity for any BioBrick module. To test whether this is the case, we are in the following months conducting a series of biological experiments investigating how our system performs in E. Coli.

In interplay with the experimental efforts, we are setting up models to test hypotheses about the system and to guide the design process. Modeling hopefully also provides a quantitative understanding useful for those who wish to employ our final BioBrick.