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Team:DTU-Denmark-2/Project/achievements
From 2011.igem.org
Achievements
Even though we first started the iGEM project in the end of June, we have managed to design a fully standardized cloning system called Plug'n Play with DNA. We think that iGEM should be about fast and easy assembling of BioBricks, which led us to use features from the USER fusion standard assembly to model our Plug'Play with DNA assembly system. Our Plug'n Play assembly consist of:
To verify the function of our design we examined various Biobriks, parts and devices, by experimental procedures described under protocols.
We have characterized the two fungal promoters, PalcA and DMKP-P6, and proved function of three mammalian promoters, SV40, PGK, CMV.
The characterization of the fungal promoters was qualitative evaluated with an X-gal analysis, where the expression of ß-galactosidase resulted in blue colonies for both PalcA and DMKP-6. Furthermore, quantitative ß-galactosidase and Bradford assay was performed to measure the protein production and ß-galactosidase activity. The activity of ß-galactosidase was highest for DMKP-6 than PalcA, which was expected to have same activity.
To demonstrate Plug' Play with DNA also was applicable in mammalian cell lines, the transfected cells capability to express the fluorescence molecules was investigated with confocal microscopy.
To ensure that there had been no occurrence of mutation and incorrect insertions in the BioBricks, they was sequenced and the resulting result was examined. The results from the sequencing supported the evaluation and characterization, implying that the system works as expected in both mammalian cells and fungi
Through this study, we have registered 70 parts.... Name part -favorite.
Medal accomplishment
Bronze
1. Team Registration
All team members are registrated
2. Complete judging form
Judgingform will be completed as soon as possible.
3. Create and share a description of the teams project using iGEM wiki and the Team's parts.
We have made a description of our project and submitted parts. can be found under Project.
4. Plan to present a poster and talk at the iGem Jamboree.
Presentation is taken form and the rehearsal for the talk at iGEM will be getting fine-tuned before the Jamboree. 5. Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Standard Biological Parts
We have submitted several new BioBricks.
Silver
1+2. Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected; characterize the operation of your new part/device. Enter tis information on the"Main Page" section of the registry
We have, among other, demonstrated the function of the device, BBa_K678002, transfected into mammalian cell lines. Furthermore, characterization of fungal promoter parts has been executed, and the results can be viewed at our homepage or in the BioBrick registry for BBa_K678000 and BBa_K678001
Gold
1:Improve an existing BioBrick Part or Device and enter this information back on the Experience Page of the Registry.
We used the CMV promoter, BBa_J52034 , as the regulator for our fluorescence reporters, and it worked well. Announced under Experience.
2: Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.
We have helped iGEM copenhagen with the design and assembly of some of their BioBricks.
3:Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.
