Team:DTU-Denmark

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== Project Description ==
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== Tuning regulation with a non-coding RNA trap ==
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Non-coding RNA is an active area of research with untapped possibilities for application in biotechnology. In prokaryotes a growing number of small RNA (sRNA) have revealed unsuspected layers of regulation.
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Small regulatory RNA is an active area of research with untapped possibilities for application in biotechnology. One such application could be the optimization and fine-tuning of synthetic biological circuits, which is currently a cumbersome process of trial and error. We have investigated a novel type of RNA regulation, where the inhibition caused by a small regulatory RNA is relieved by another RNA called trap-RNA. The system displays a large dynamic range and can uniquely target and repress any gene of interest providing unprecedented flexibility. We suspect that any level of repression is achievable by simply altering the sequences of the involved RNAs. Multiple such systems can coexist without interfering and are thus compatible with more complex designs. Furthermore the trap-RNA can be fused to any transcript in effect allowing any gene to act as an activator.
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We at the iGEM DTU-Denmark team are designing an sRNA based regulatory system compatible with the BioBrick standard. Our core ideas are inspired by a novel mechanism of sRNA mediated post-transcriptional regulation found in E. Coli and Salmonella. The naturally occurring system consists of a regulation topology where one sRNA inhibits another sRNA, which in turn inhibits a third RNA that is translated. The inhibitions might be codable on the sequence level and therefore the system may provide design solutions enabling efficient regulatory control and specificity towards any BioBrick module. To test whether this is the case, we are constructing a biological E. Coli model enabling us to control the synthesis of both sRNAs and measure the outcome of the regulatory system.
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In interplay with the experimental efforts, we are setting up models to test hypotheses about the system and to guide the design process. Modeling should also provide a quantitative understanding useful for those who wish to employ our final BioBrick.
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== Remember! ==
== Remember! ==

Revision as of 13:31, 12 September 2011


Tuning regulation with a non-coding RNA trap

Small regulatory RNA is an active area of research with untapped possibilities for application in biotechnology. One such application could be the optimization and fine-tuning of synthetic biological circuits, which is currently a cumbersome process of trial and error. We have investigated a novel type of RNA regulation, where the inhibition caused by a small regulatory RNA is relieved by another RNA called trap-RNA. The system displays a large dynamic range and can uniquely target and repress any gene of interest providing unprecedented flexibility. We suspect that any level of repression is achievable by simply altering the sequences of the involved RNAs. Multiple such systems can coexist without interfering and are thus compatible with more complex designs. Furthermore the trap-RNA can be fused to any transcript in effect allowing any gene to act as an activator.

Remember!

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