Team:Cambridge

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         <p>Abstract</p>
         <p>Abstract</p>
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       <p>We investigated the properties of a novel yet under-researched group of proteins called reflectins.</p>
       <p>We investigated the properties of a novel yet under-researched group of proteins called reflectins.</p>
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       <p>Reflectins are interesting because of their self-organising properties, which cause them to produce dynamic structural colour.</p>
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       <p>Reflectins are interesting and unusual because of their self-organising properties, which cause them to produce dynamic structural colour.</p>
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       <p>We expressed codon-optimised reflectin in E.Coli, and observed the protein's in vivo and in vitro effects.</p>
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       <p>We expressed codon-optimised reflectin in E. coli, and investigated the proteins' in vivo and in vitro effects.</p>
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         <p>Structural Colour</p>
         <p>Structural Colour</p>
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       <p>Nature’s colours don’t just come from pigments, but from structure too.</p>
       <p>Nature’s colours don’t just come from pigments, but from structure too.</p>
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         <p>Impact of iGEM</p>
         <p>Impact of iGEM</p>
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        <p>We wanted to know what effect the iGEM competition has had on its past contestants.</p>
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      <p>We wanted to know whether the iGEM has had any effect on it's previous contestants.</p>
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        <p>We were also interested in what past participants thought of iGEM and how it shaped their view of synthetic biology.</p>
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      <p>To this end, we conducted a number of interviews with previous iGEMmers, as well as conducting a simple poll of previous contestants.</p>
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        <p>We got in touch with a number of iGEM alumni and asked them to share their experience.</p>
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         <p>Tools</p>
         <p>Tools</p>
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        <p>We used Gibson Assembly exclusively to build our constructs during the competition. This allowed us to do multi-part assemblies faster and with fewer bugs than if we had used standard techniques.</p>
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      <p>We used Gibson Assembly instead exclusively for construct assembly during the competition, allowing us to assemble plasmids faster and with greater ease than would be possible using standard techniques.</p>
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        <p>We contributed to a collection of software tools called <i>Gibthon</i> (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.</p>
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      <p>We contributed to a collection of software tools called <a href='http://www.gibthon.org'>Gibthon</a> (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.</p>
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