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A Chassis Using Native Tailings Pond Species

Pseudomonas (left), microalgae species Dunaliella tertiolecta (right).

Chassis Selection

We identified early on in our project planning stages that we would like to use Pseudomonas or microalgae as the chassis to house our system. The motivation for this is that not only are these organisms capable of naturally surviving a tailings pond environment, but they may contain necessary regulatory elements required for a response to NAs. Because of this, taking a response element out of an organism and putting our system into another chassis, such as E. coli, may not behave as expected. Additionally, we characterized a change in growth rate of E. coli with and without tailing ponds showing that, cells don't grow as well in this media (This work was done in collaberation with the Lethbridge team - See our collaboration section for more information on this research). Based on this chassis choice however, we realized that given their scarcity in iGEM, tools were needed to establish work in these organisms for future iGEM teams.

Click here to read more about our Microalgae Tools.

Click here to read more about our Pseudomonas Tools.