Revision as of 20:24, 28 September 2011 by Rpgguardian (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

• Home
• Team
  • Biographies
  • Facilitators
  • The University
  • Official Profile
• Project
  • Overview
  • Promoter
  • Reporter
  • Chassis
  • Prototype
  • Data Page
  • Accomplishments
  • Future Directions
  • References
• Parts
  • Parts Submitted
  • Attributions
• Notebook
  • Post-Regionals
  • Journal
  • Protocols
  • Safety
• Outreach
  • Human Practices
  • Conferences
  • Follow Us!
• Sponsors
  • Sponsors
  • Acknowledgements
• iGEM

• Overview
• Promoter Project
  • Sensory Element Fishing
  • Bioinformatics and RT-PCR
• Reporter Project
  • Preliminary Testing
  • Characterization
  • Optimization
• Chassis Project
• Microalgae Tools
• Pseudomonas Tools
• Prototype
• Accomplishments
• Data Page
• Future Directions
• References

The University of Calgary iGEM Team has had many accomplishments this Summer

In our promoter project...

• We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.
• We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecuels to screen for interactors in the search for responsive elements.
• We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.


In our Reporter Project...

• We characterized a novel reporter function for the lacZ gene using an electrochemical output.
• We laid the groundwork for future use of electrochemical reporting in iGEM


In our Chassis project...

• We characterized a Pseudomonas-E. coli conjugation construct
• We submitted parts and protocols for future work in both microalgae and Pseudomonas
• We characterized the viability of Pseudomonas and E. coli to grow and survive in tailings ponds

Judging Requirements

Bronze:

• Team registration - Our team successfully registered for the iGEM Competition
• Complete Judging Form - To view our Judging Form please href="https://igem.org/2011_Judging_Form?id=640" click here
• Team Wiki - Our team has successfully designed a wiki page for our project
• Submit at least one new well-characterized standard BioBrick Part - Our team has submitted several new parts all of which we have fully characterized.

Silver:

• Demonstrate at least one new BioBrick part designed works as expected - All of our parts have been carefully tested. Our oriT-ori1600 Psuedomonas conjugation part has been well characterized.

Gold: Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year) and enter this information in the Registry (in the “Experience” section of that BioBrick’s Registry entry), and don't forget to create a new registry page for the improved part. The growth of the Registry depends on having a broad base of reliable parts. This is why the improvement of an existing part is just as important as the creation and documentation of a new part. An "improvement" is anything that improves the functionality and ease-of-use of a part, so that it is more likely to be used by the community. For instance: strengthening the expression of a part by mutating the DNA sequence; modifying one or a few parts in construct (Device) so that it performs its intended job better; improving a cloning or expression vector that can be easily used by the entire community; and of course, troubleshooting and fixing a part reported to be non-functional. Data from an experimental comparison between the original and improved part/ device is strongly recommended. Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.