Team:Calgary/Project/Acomplishments

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Accomplishments

The University of Calgary iGEM Team has had many accomplishments this Summer

In our promoter project...
  • We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.
  • We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecuels to screen for interactors in the search for responsive elements.
  • We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.


  • In our Reporter Project...
  • We characterized a novel reporter function for the lacZ gene using an electrochemical output.
  • We laid the groundwork for future use of electrochemical reporting in iGEM


  • In our Chassis project...
  • We characterized a Pseudomonas-E. coli conjugation construct
  • We submitted parts and protocols for future work in both microalgae and Pseudomonas
  • We characterized the viability of Pseudomonas and E. coli to grow and survive in tailings ponds