Team:Calgary/Project/Acomplishments

From 2011.igem.org

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<b>The University of Calgary iGEM Team has had many accomplishments this Summer</b><br><br>
<b>The University of Calgary iGEM Team has had many accomplishments this Summer</b><br><br>
<b>In our promoter project...</b><br>
<b>In our promoter project...</b><br>
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We designed a novel protocol to identify small hydrophobic molecule interacters. <br>
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<ul><li>We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.</li>
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We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecuels to screen for interactors in the search for sensory elements. <br>
+
<li>We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecuels to screen for interactors in the search for responsive elements.</li>
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We identified a gene that is upregulated in the presence of NA's, suggesting a possible promoter regions.<br><br>
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<li>We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.</li><br><br>
<b>In our Reporter Project...</b><br>
<b>In our Reporter Project...</b><br>
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We characterized a novel reporter function for the lacZ gene using an electrochemical output.<br><br>
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<li>We characterized a novel reporter function for the lacZ gene using an electrochemical output.</li>
 +
<li>We laid the groundwork for future use of electrochemical reporting in iGEM</li><br><br>
<b>In our Chassis project...</b><br>
<b>In our Chassis project...</b><br>
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We characterized a Pseudomonas-E. coli conjugation construct<br>
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<li>We characterized a<i> Pseudomonas-E. coli</i> conjugation construct</li>
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Submitted parts and protocols for future work in microalgae and Pseudomonas
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<li>We submitted parts and protocols for future work in both microalgae and <i>Pseudomonas</i></li>
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<li>We characterized the viability of <i>Pseudomonas</i> and <i>E. coli</i> to grow and survive in tailings ponds</li>
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Revision as of 18:01, 28 September 2011


Accomplishments

The University of Calgary iGEM Team has had many accomplishments this Summer

In our promoter project...
  • We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.
  • We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecuels to screen for interactors in the search for responsive elements.
  • We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.


    • In our Reporter Project...
  • We characterized a novel reporter function for the lacZ gene using an electrochemical output.
  • We laid the groundwork for future use of electrochemical reporting in iGEM


    • In our Chassis project...
  • We characterized a Pseudomonas-E. coli conjugation construct
  • We submitted parts and protocols for future work in both microalgae and Pseudomonas
  • We characterized the viability of Pseudomonas and E. coli to grow and survive in tailings ponds

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