Team:Calgary/Notebook/Protocols/Process4

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TITLE=qRT-PCR| BODY=

We have a mastermix of PCR enzyme, sybr green, and dNTPs that is 2x. Primers will be stored at 100μM and used at final concentrations between 200-600nM. The PCR mix will be as follows:

qRT-PCR using Quanta PCR mastermix

Component Volume
Quanta PCR master mix 5 uL
Primers (Fwd&Rev) at 600nM each 3 uL
cDNA 2 uL
Final Volume 10 uL

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