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Team:Calgary/Notebook/Protocols/Process4
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Emily Hicks (Talk | contribs) (Created page with "{{Team:Calgary/Main_Header|notebook}} {{Team:Calgary/Notebookbar| TITLE=qRT-PCR| BODY=<html> <p>We have a mastermix of PCR enzyme, sybr green, and dNTPs that is 2x. Primers w...") |
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</tr> | </tr> | ||
| + | </table> | ||
| + | <p>The following cylcing conditions are used.</p> | ||
| + | |||
| + | <div style="margin-bottom:60px"> | ||
| + | <table width="700"> | ||
| + | <tr> | ||
| + | <td width="85%"><div class="heading"><p style="font-size:14px; font-weight:bold"><a style="text-decoration:none" name="reaction conditions"></a> </p></div></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table width="630" border="1px" style="margin-bottom:15px;"> | ||
| + | <tr> | ||
| + | <td><CENTER>2 min at 50°C</CENTER></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><CENTER>10 min at 95°C</CENTER></td> | ||
| + | </tr> | ||
| + | <tr><td><b>40 cycles of:</b></tr></td> | ||
| + | <tr> | ||
| + | <td><CENTER>15 sec at 95°C</CENTER></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><CENTER>1 min at 60°C</CENTER></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>Melting curve analysis</b></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><CENTER>15 sec at 95°C</CENTER></td> | ||
| + | </tr> | ||
</table> | </table> | ||
Revision as of 20:54, 27 September 2011
TITLE=qRT-PCR| BODY=
We have a mastermix of PCR enzyme, sybr green, and dNTPs that is 2x. Primers will be stored at 100μM and used at final concentrations between 200-600nM. The PCR mix will be as follows:
