http://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Protocols/Process28&feed=atom&action=historyTeam:Calgary/Notebook/Protocols/Process28 - Revision history2024-03-28T13:32:48ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Protocols/Process28&diff=202721&oldid=prevEmily Hicks at 03:53, 29 September 20112011-09-29T03:53:49Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:53, 29 September 2011</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>TITLE=<del class="diffchange diffchange-inline">testing </del>For Oxidation|</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>TITLE=<ins class="diffchange diffchange-inline">Testing </ins>For Oxidation|</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>For <del class="diffchange diffchange-inline">the electrodes we used the Screen printed Carbon Electrodes from Pine Instruments. These electrodes needed to be plated in zinc phthalocyanine for our purposes.</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>For <ins class="diffchange diffchange-inline">this procedure you will need:</ins></p><br<ins class="diffchange diffchange-inline">><ul</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">ol</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">li>Freshly prepared buffer solution</li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><del class="diffchange diffchange-inline">Dissolve 100µg of zinc phthalocyanine in 10mL of DMF.</del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><ins class="diffchange diffchange-inline">Solution to be measured</ins></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><del class="diffchange diffchange-inline">Stir until dissolved.</del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><ins class="diffchange diffchange-inline">Freshly plated electrode</ins></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><del class="diffchange diffchange-inline">Dip only </del>the <del class="diffchange diffchange-inline">working </del>electrode <del class="diffchange diffchange-inline">(outer </del>electrode<del class="diffchange diffchange-inline">) </del>into the <del class="diffchange diffchange-inline">solution, and hold it there for 5 seconds.</del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><ins class="diffchange diffchange-inline">Potentiostat with electrode adapter</li></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><del class="diffchange diffchange-inline">Let </del>the electrode <del class="diffchange diffchange-inline">air dry until there is no more </del>solution <del class="diffchange diffchange-inline">present on the electrode.</del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>AfterMath installed on a computer</li></ul><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">/ol</del>><<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>This procedure will show oxidization of a compound and can generate standard curves. A picture of </ins>the <ins class="diffchange diffchange-inline">potentiostat and connected </ins>electrode <ins class="diffchange diffchange-inline">is shown at the bottom of the page.</p><br><ol></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">p</del>><<del class="diffchange diffchange-inline">b</del>><del class="diffchange diffchange-inline">NOTE</del></<del class="diffchange diffchange-inline">b</del>>: <del class="diffchange diffchange-inline">This should be performed in a fume hood due to the toxic nature of DMF</del></<del class="diffchange diffchange-inline">p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>Place the plated </ins>electrode into the <ins class="diffchange diffchange-inline">adapter (the use of a blank spacer may be needed)</ins></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><ins class="diffchange diffchange-inline">Place </ins>the <ins class="diffchange diffchange-inline">connected </ins>electrode <ins class="diffchange diffchange-inline">into the buffer </ins>solution <ins class="diffchange diffchange-inline">and take a blank reading through AfterMath</ins></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">li</ins>><ins class="diffchange diffchange-inline">Add the solution to be measured and test for oxidation through AfterMath</ins><<ins class="diffchange diffchange-inline">/li</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">li</ins>><ins class="diffchange diffchange-inline">Dispose of all solutions and electrodes according to the policies in your facility</ins><<ins class="diffchange diffchange-inline">/li</ins>></<ins class="diffchange diffchange-inline">ol</ins>><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img style="width</ins>:<ins class="diffchange diffchange-inline">250px; display:block; margin-left:auto; margin-right:auto;" src="https://static.igem.org/mediawiki/2011/5/5b/UofC_ElecSetup.jpg"></ins></<ins class="diffchange diffchange-inline">img</ins>></div></td></tr>
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</table>Emily Hickshttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Protocols/Process28&diff=200691&oldid=prevEmily Hicks: Created page with "{{Team:Calgary/Main_Header|notebook}} {{Team:Calgary/Notebookbar| TITLE=testing For Oxidation| BODY=<html> <p>For the electrodes we used the Screen printed Carbon Electrodes fro..."2011-09-29T02:50:37Z<p>Created page with "{{Team:Calgary/Main_Header|notebook}} {{Team:Calgary/Notebookbar| TITLE=testing For Oxidation| BODY=<html> <p>For the electrodes we used the Screen printed Carbon Electrodes fro..."</p>
<p><b>New page</b></p><div>{{Team:Calgary/Main_Header|notebook}}<br />
{{Team:Calgary/Notebookbar|<br />
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TITLE=testing For Oxidation|<br />
BODY=<html><br />
<p>For the electrodes we used the Screen printed Carbon Electrodes from Pine Instruments. These electrodes needed to be plated in zinc phthalocyanine for our purposes.</p><br><br />
<ol><br />
<li>Dissolve 100µg of zinc phthalocyanine in 10mL of DMF.</li><br />
<li>Stir until dissolved.</li><br />
<li>Dip only the working electrode (outer electrode) into the solution, and hold it there for 5 seconds.</li><br />
<li>Let the electrode air dry until there is no more solution present on the electrode.</li><br />
</ol><br><br />
<p><b>NOTE</b>: This should be performed in a fume hood due to the toxic nature of DMF</p><br />
</html><br />
}}</div>Emily Hicks