Team:Calgary/Notebook/Protocols/Process14

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Team:Calgary/Main_Header|notebook}} {{Team:Calgary/Notebookbar| TITLE=Rehydration of Registry DNA| BODY=<html> <tr> <td> <div style="margin-bottom:60px"> <table width="700"...")
 
(9 intermediate revisions not shown)
Line 5: Line 5:
BODY=<html>
BODY=<html>
-
<tr>
+
   <p> Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used. </p>
-
<td>
+
-
<div style="margin-bottom:60px">
+
-
  <table width="700">
+
-
    <tr>
+
-
      <td width="85%"><div class="heading"><a style="text-decoration:none" name="rehydration"></a> Rehydration</div></td>
+
-
      <td width="15%"><a style="float:right;" href="#top" title="return to top">return to top</a> </td>
+
-
    </tr>
+
-
  </table>
+
-
   <p> Biobrick parts are shipped from the registry in a dehydrated from. As such they must be rehydrated before they can be used. </p>
+
   <ol>
   <ol>
     <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
     <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
-
     <li> Add 15 μL of diH20 (deionized water)</li>
+
     <li> Add 10 μL of ddH<sub>2</sub>O (deionized water)</li>
-
     <li> Let the water sit for 5 minutes</li>
+
     <li> Let the water incubate at room temperature for 10 minutes</li>
-
     <li>Take 2 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies</li>
+
     <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.</li>
   </ol>
   </ol>
-
</div>
+
<br></br><br></br><br></br><br></br><br></br><br></br><br></br>
-
<br>
+
 
-
</td>
+
 
-
</tr>
+
</html>
 +
}}

Latest revision as of 04:19, 29 September 2011


Rehydration of Registry DNA

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.

  1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
  2. Add 10 μL of ddH2O (deionized water)
  3. Let the water incubate at room temperature for 10 minutes
  4. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.














Retrieved from "http://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14"