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Team:Calgary/Notebook/Protocols/Process14
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| - | <p> Biobrick parts are shipped from the registry in a dehydrated | + | <p> Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used. </p> |
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<li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> | <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> | ||
Revision as of 21:33, 27 September 2011
TITLE=Rehydration of Registry DNA| BODY=
Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.
- Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
- Add 10 μL of ddH20 (deionized water)
- Let the water incubate at room temperature for 10 minutes
- Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies
