Team:Calgary/Notebook/Protocols/Process10

The kit we used over the summer was purchased from Qiagen. This kit is used to purify the PCR product.

1. Add 5 volumes of Buffer PB to 1 volume of PCR product and then mix.
2. Place a spin column in a 2 mL collection tube.
3. Apply the mixture of PB buffer + PCR product through the QIA column and centrifuge for 1 minute.
4. Discard the flow-through and place the column back in the tube.
5. Wash the product using 0.75 mL buffer PE and then centrifuge for 1 min.
6. Discard the flow-through and place the column back into the tube.
Centrifuge the column inside the tube to discard the additional fluid in the column.
7. Elute the DNA into a clean microcentrifuge tube with buffer EB.