"
Team:Calgary/Notebook/Protocols/Process10
From 2011.igem.org
(Difference between revisions)
Emily Hicks (Talk | contribs) (Created page with "{{Team:Calgary/Main_Header|notebook}} {{Team:Calgary/Notebookbar| TITLE=cDNA Synthesis/Reverse Transcription Reaction| BODY=<html> <tr> <td> <div style="margin-bottom:60px"> <...") |
Emily Hicks (Talk | contribs) |
||
| Line 14: | Line 14: | ||
</table> | </table> | ||
<p>The kit we used over the summer was purchased from Qiagen. This kit is | <p>The kit we used over the summer was purchased from Qiagen. This kit is | ||
| - | used to purify | + | used to purify PCR products, as well DNA products in enzymatic reactions, particularly, restriction digests. |
</p> | </p> | ||
<ol> | <ol> | ||
Revision as of 21:00, 27 September 2011
| return to top |
The kit we used over the summer was purchased from Qiagen. This kit is used to purify PCR products, as well DNA products in enzymatic reactions, particularly, restriction digests.
- Add 5 volumes of Buffer PB to 1 volume of PCR product and then mix.
- Place a spin column in a 2 mL collection tube.
- Apply the mixture of PB buffer + PCR product through the QIA column and centrifuge for 1 minute.
- Discard the flow-through and place the column back in the tube.
- Wash the product using 0.75 mL buffer PE and then centrifuge for 1 min.
- Discard the flow-through and place the column back into the tube. Centrifuge the column inside the tube to discard the additional fluid in the column.
- Elute the DNA into a clean microcentrifuge tube with buffer EB.
