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Team:Calgary/Notebook/Protocols
From 2011.igem.org
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD Construct</a></li> | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD-LacZ Construct</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal transformation</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal transformation</a></li> | ||
Revision as of 23:19, 27 September 2011
Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Protocols
Hello! Our wiki is currently under heavy construction, so please check regularly for updates. Thank you for your patience.
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Plasmid Extraction
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-LacZ Construct
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the LacI-LacZ I732901 gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing chemically competent cells (E. coli)
- Preparing glycerol stocks (E. coli)
