Entry #3: the Alberta iGEM

Every year, the Albertan iGEM teams from Lethbridge, Edmonton, and Calgary converge for several conferences collectively called "aGEM". This weekend, we had the first aGEM and got to meet the members from Lethbridge and Edmonton. We also heard about their projects, the challenges they faced, and met with several speakers to discuss the personal, technical, and communication challenges ahead. Later, we took a tour of Peter's laboratory, where we encountered a mass spectronomer, lots of fancy equipment ridden with danger warnings, and an opium lab.

Lethbridge's Project

Last year, Lethbridge designed a strain of e.coli to degrade corticol, a type of bone tissue. Though successful, the strain was not very efficient. This year's project is to optimize it; a major focus is on the development of micro-compartments and target breakdown pathways. Compartments are for keeping complementary enzymes close together, and current figures suggest they can contain up to 5 luminescence proteins.

Edmonton's Project

Edmonton wants to modify bread mold to break down common industrial waste products such as saw-dust into usable fuels, like biodiesel. The key feature of the fungus is that it grows well on cellulose; Edmonton's delegate, Rae, named four key goals in their project.

• Find the ideal growth conditions for the mold in environments consisting of industrial waste products, such as wheatstraw, sawdust, etc.
• Modify genes to "jack up" fatty acids.
• Synthesize biodiesel efficiently using the fatty acids produced by the fungus.
• Integrate the fungus into a self-contained bio-reactor for industrial and home use.

The team anticipates the major challenge will be working with the genome of the fungus. The fungus' unusual genome makes it difficult to transform, and over 70 proteins are involved in growing on cellulose, making the insertion and extraction of genes a highly elaborate task.

The Guest Speakers

We had five guest speakers: Samantha Sutton, Michael Mader, Peter Facchini, Lorri, and Ann. The first four guest speakers took preliminary questions as a panel, and then separately led a workshop for each team. Michael and Lorri talked about the legal, ethical, and political implications of our project, and Peter did the technical parts; Samantha gave us some guidance in becoming a stronger team, working well together, and communicating important details.