http://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&feed=atom&action=historyTeam:Calgary/Notebook/Calendar/LiveCell - Revision history2024-03-29T01:49:04ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=203777&oldid=prevPjwu at 04:45, 29 September 20112011-09-29T04:45:04Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2011/b/bc/UCalgary2011_Arabinose_cprg_graph.png" width="660px"></img></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img <ins class="diffchange diffchange-inline">style="width: 580px;" </ins>src="https://static.igem.org/mediawiki/2011/b/bc/UCalgary2011_Arabinose_cprg_graph.png" width="660px"></img></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b>Figure 1.</b> OD readings measuring the production of PCR by the cleavage of CPRG by β-galactosidase expressed by <i>E. coli</i> over a range of time</p><br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b>Figure 1.</b> OD readings measuring the production of PCR by the cleavage of CPRG by β-galactosidase expressed by <i>E. coli</i> over a range of time</p><br></br></div></td></tr>
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</table>Pjwuhttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=203749&oldid=prevEmily Hicks at 04:43, 29 September 20112011-09-29T04:43:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>I tried transforming TOP10 cells with I723032 from the 2008 registry parts however days after plating on LB Agar, no growth was observed.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>I tried transforming TOP10 cells with I723032 from the 2008 registry parts however days after plating on LB Agar, no growth was observed.</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>The <del class="diffchange diffchange-inline">ORI</del>-T/R project was described to me.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>The <ins class="diffchange diffchange-inline">ori</ins>-T/R project was described to me.</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>I with the help of Robert and Emily, discovered that the concentration of NAs that I had been exposing the E. coli in my three previous viability assays was in fact 1000 times higher than I had thought.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>I with the help of Robert and Emily, discovered that the concentration of NAs that I had been exposing the<ins class="diffchange diffchange-inline"><i> </ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>in my three previous viability assays was in fact 1000 times higher than I had thought.</li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>RESULTS</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>RESULTS</h4></div></td></tr>
</table>Emily Hickshttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=203723&oldid=prevEmily Hicks at 04:40, 29 September 20112011-09-29T04:40:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Tuesday, July 12, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Tuesday, July 12, 2011</h4></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Spent the day going over all previous gels numbering the lanes and making legends. Also consolidated the images/results from the E. coli assay. Overnight cultures were set-up from the E. coli strains carrying the three constructs created the previous week.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Spent the day going over all previous gels numbering the lanes and making legends. Also consolidated the images/results from the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>assay. Overnight cultures were set-up from the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>strains carrying the three constructs created the previous week.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 13, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 13, 2011</h4></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Thursday, July 14, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Thursday, July 14, 2011</h4></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> Discussed and planned out another E. coli viability/survival assay in NAs and made some autoclaved PBS buffer in preparation for it. Also according to the results of the gel from the previous day it was decided that the constructs made previously would have to be remade perhaps using other parts. Some more of the previous gels were also labelled in preparation for being posted on the website. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p> Discussed and planned out another <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>viability/survival assay in NAs and made some autoclaved PBS buffer in preparation for it. Also according to the results of the gel from the previous day it was decided that the constructs made previously would have to be remade perhaps using other parts. Some more of the previous gels were also labelled in preparation for being posted on the website. </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Tuesday, July 19, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Tuesday, July 19, 2011</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In Preparation for the E. coli viability assay that was to be performed the next day the strain carrying the I0500_B0034_I732005 insert was cultured. The purpose of the assay was to determine whether or not the E. Coli could survive in the presence of NAs and therefore whether it would be practical to use it in the final biosensor. On this particular occasion the NAs were diluted in autoclaved PBS buffer rather than ddH2O, mainly because it was theorized that the inability of the cells to survive in the previous assays was because of the hypotonicity of the water. On a different note some TOP10 cells were transformed for Emily.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In Preparation for the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>viability assay that was to be performed the next day the strain carrying the I0500_B0034_I732005 insert was cultured. The purpose of the assay was to determine whether or not the <ins class="diffchange diffchange-inline"><i></ins>E. Coli<ins class="diffchange diffchange-inline"></i> </ins>could survive in the presence of NAs and therefore whether it would be practical to use it in the final biosensor. On this particular occasion the NAs were diluted in autoclaved PBS buffer rather than ddH2O, mainly because it was theorized that the inability of the cells to survive in the previous assays was because of the hypotonicity of the water. On a different note some TOP10 cells were transformed for Emily.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 20, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 20, 2011</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> Performed the E. coli viability assay, which took most of the day. Also I did some reading on electrochemical sensors</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p> Performed the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>viability assay, which took most of the day. Also I did some reading on electrochemical sensors</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Friday, July 22, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Friday, July 22, 2011</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>I went over the results of the E. coli assay from earlier in the week and concluded that our strain of E. coli (Invitrogen TOP10) was not a suitable candidate in use for the biosensor, because the <del class="diffchange diffchange-inline">e</del>.coli could not survive sufficient levels of naphthenic acids.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>I went over the results of the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>assay from earlier in the week and concluded that our strain of <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>(Invitrogen TOP10) was not a suitable candidate in use for the biosensor, because the <ins class="diffchange diffchange-inline"><i>E</ins>.coli<ins class="diffchange diffchange-inline"></i> </ins>could not survive sufficient levels of naphthenic acids.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- Include pictures for this week--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- Include pictures for this week--></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>I ran a colony PCR on the cells transformed the previous day and on a streak plate of the cells carrying the contruct I0500_B0034_I732005.I subcultured the culture from the previous day until it reached log phase and then incubated the cells in CPRG and arabinose and monitored the response. The results of the experiment indicated that the cells were converting CPRG into CPR in the presence of arabinose. Details of the experiment are below:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>I ran a colony PCR on the cells transformed the previous day and on a streak plate of the cells carrying the contruct I0500_B0034_I732005.I subcultured the culture from the previous day until it reached log phase and then incubated the cells in CPRG and arabinose and monitored the response. The results of the experiment indicated that the cells were converting CPRG into CPR in the presence of arabinose. Details of the experiment are below:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>I performed a test on E.coli carrying the I0500_B0034_I732005 (arabinose promoter followed by LacZ reporter) to measure the conversion of CPRG into CPR. The experiment was not kept buffered at a constant pH of 7 at which pH the CPR would have have very good absorbance. Near the end though the pH was 7 presumably because the E.coli would have produced their own buffer and increased the pH with it. At different pH's though, the solution has different optical density because of the indicator (CPR) used in the experiment. So the results may not be indicative of the level of CPR production over time. The results are below</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>I performed a test on <ins class="diffchange diffchange-inline"><i></ins>E.coli<ins class="diffchange diffchange-inline"></i> </ins>carrying the I0500_B0034_I732005 (arabinose promoter followed by LacZ reporter) to measure the conversion of CPRG into CPR. The experiment was not kept buffered at a constant pH of 7 at which pH the CPR would have have very good absorbance. Near the end though the pH was 7 presumably because the <ins class="diffchange diffchange-inline"><i></ins>E.coli<ins class="diffchange diffchange-inline"></i> </ins>would have produced their own buffer and increased the pH with it. At different pH's though, the solution has different optical density because of the indicator (CPR) used in the experiment. So the results may not be indicative of the level of CPR production over time. The results are below</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2011/b/bc/UCalgary2011_Arabinose_cprg_graph.png" width="660px"></img></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2011/b/bc/UCalgary2011_Arabinose_cprg_graph.png" width="660px"></img></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><b>Figure 1.</b> OD readings measuring the production of PCR by the cleavage of CPRG by β-galactosidase expressed by E. coli over a range of time</p><br></br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><b>Figure 1.</b> OD readings measuring the production of PCR by the cleavage of CPRG by β-galactosidase expressed by <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>over a range of time</p><br></br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 27, 2011.</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 27, 2011.</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>I ran a gel to determine the results from the PCR performed the previous day, it turned out that the PCR was a failure. I retried the PCR again, this time using a different recipe for the mastermix. On a different note I sonicated some <del class="diffchange diffchange-inline">pseudomonas </del>cells which had been lysed to try to get the genome cut into units of 1KB, however a gel ran by Emily determined that the sonication failed to do so.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>I ran a gel to determine the results from the PCR performed the previous day, it turned out that the PCR was a failure. I retried the PCR again, this time using a different recipe for the mastermix. On a different note I sonicated some <ins class="diffchange diffchange-inline"><i>Pseudomonas</i> </ins>cells which had been lysed to try to get the genome cut into units of 1KB, however a gel ran by Emily determined that the sonication failed to do so.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Thursday, July 28, 2011.</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Thursday, July 28, 2011.</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>I ran a gel which confirmed the success of the PCR of the previous day. R0010 was successfully transformed into the E. coli cells, however the gel did not indicate any bands in the region of 1200KB indicating that either the 2011 registry plate did actually contain I0500 DNA in the well that was supposed to contain it or that consistently we were not able to transform bacteria with I0500 from the 2011 registry plates. Also I cultured one of the bacterial colonies carrying R0010 (confirmed by PCR). Later in the day I sonicated samples of lysed E. coli and <del class="diffchange diffchange-inline">pseudomonas </del>for Emily.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>I ran a gel which confirmed the success of the PCR of the previous day. R0010 was successfully transformed into the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>cells, however the gel did not indicate any bands in the region of 1200KB indicating that either the 2011 registry plate did actually contain I0500 DNA in the well that was supposed to contain it or that consistently we were not able to transform bacteria with I0500 from the 2011 registry plates. Also I cultured one of the bacterial colonies carrying R0010 (confirmed by PCR). Later in the day I sonicated samples of lysed <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>and<ins class="diffchange diffchange-inline"><i> Pseudomonas</i> </ins>for Emily.</p></div></td></tr>
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</table>Emily Hickshttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=203669&oldid=prevEmily Hicks at 04:36, 29 September 20112011-09-29T04:36:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Project Description </h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Project Description </h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The purpose of this project was to identify a suitable reporter gene for the electrochemical detector, to characterize it and to make a construct that included both an easily inducible promoter such as pBAD and the reporter. Ultimately the reporter that was decided on was LacZ and the IPTG inducible promoter was decided on as opposed to pBAD for the purposes of testing the LacZ gene and characterizing it as an electrochemical reporter. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The purpose of this project was to identify a suitable reporter gene for the electrochemical detector, to characterize it and to make a construct that included both an easily inducible promoter such as pBAD and the reporter. Ultimately the reporter that was decided on was <ins class="diffchange diffchange-inline"><i></ins>LacZ<ins class="diffchange diffchange-inline"></i> </ins>and the IPTG inducible promoter was decided on as opposed to pBAD for the purposes of testing the<ins class="diffchange diffchange-inline"><i> </ins>LacZ<ins class="diffchange diffchange-inline"></i> </ins>gene and characterizing it as an electrochemical reporter. </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3> Project Participants</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3> Project Participants</h3></div></td></tr>
</table>Emily Hickshttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=200292&oldid=prevMyarcell at 02:34, 29 September 20112011-09-29T02:34:46Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>TITLE = <del class="diffchange diffchange-inline">Live Cell Design </del>Project |</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>TITLE = <ins class="diffchange diffchange-inline">Reporter Gene </ins>Project |</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BODY = </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BODY = </div></td></tr>
</table>Myarcellhttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=200196&oldid=prevMyarcell at 02:30, 29 September 20112011-09-29T02:30:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Tuesday, July 12, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Tuesday, July 12, 2011</h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Spent the day going over all previous gels numbering the lanes and making legends. Also consolidated the images/results from the E. coli assay. Overnight cultures were <del class="diffchange diffchange-inline">prepared </del>from the E. coli strains carrying the three constructs created the previous week.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Spent the day going over all previous gels numbering the lanes and making legends. Also consolidated the images/results from the E. coli assay. Overnight cultures were <ins class="diffchange diffchange-inline">set-up </ins>from the E. coli strains carrying the three constructs created the previous week.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 13, 2011</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Wednesday, July 13, 2011</h4></div></td></tr>
</table>Myarcellhttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=200065&oldid=prevEmily Hicks at 02:26, 29 September 20112011-09-29T02:26:28Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>TITLE = <del class="diffchange diffchange-inline">LIVE CELL DESIGN PROJECT </del>|</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>TITLE = <ins class="diffchange diffchange-inline">Live Cell Design Project </ins>|</div></td></tr>
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</table>Emily Hickshttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=199957&oldid=prevMyarcell at 02:23, 29 September 20112011-09-29T02:23:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Project Description </h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Project Description </h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The purpose of this project was to identify a suitable reporter gene for the electrochemical detector, to characterize it and to make a construct that included both an easily inducible promoter such as pBAD and the reporter. Ultimately <del class="diffchange diffchange-inline">the </del>the reporter that was decided on was LacZ and the IPTG inducible promoter was decided on as opposed to pBAD for the purposes of testing the LacZ gene and characterizing it as an electrochemical reporter. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The purpose of this project was to identify a suitable reporter gene for the electrochemical detector, to characterize it and to make a construct that included both an easily inducible promoter such as pBAD and the reporter. Ultimately the reporter that was decided on was LacZ and the IPTG inducible promoter was decided on as opposed to pBAD for the purposes of testing the LacZ gene and characterizing it as an electrochemical reporter. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3> Project Participants</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3> Project Participants</h3></div></td></tr>
</table>Myarcellhttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=190044&oldid=prevSaeedq at 07:45, 28 September 20112011-09-28T07:45:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Project Description </h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Project Description </h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> <del class="diffchange diffchange-inline">TO THE LOBBE!!!!!!!!!!!! i mean lab </del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">The purpose of this project was to identify a suitable reporter gene for the electrochemical detector, to characterize it and to make a construct that included both an easily inducible promoter such as pBAD and the reporter. Ultimately the the reporter that was decided on was LacZ and the IPTG inducible promoter was decided on as opposed to pBAD for the purposes of testing the LacZ gene and characterizing it as an electrochemical reporter. </ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3> Project Participants</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3> Project Participants</h3></div></td></tr>
</table>Saeedqhttp://2011.igem.org/wiki/index.php?title=Team:Calgary/Notebook/Calendar/LiveCell&diff=189779&oldid=prevSj.dixon at 07:12, 28 September 20112011-09-28T07:12:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!--SAEED123 - put in the graph if you wish--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!--SAEED123 - put in the graph if you wish--></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><table width="<del class="diffchange diffchange-inline">300px</del>" border="<del class="diffchange diffchange-inline">0.5</del>"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><table width="<ins class="diffchange diffchange-inline">200px</ins>" border="<ins class="diffchange diffchange-inline">1</ins>"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><th> Time(hours)</th> <th>Optical Density Reading</th></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><th> Time(hours)</th> <th>Optical Density Reading</th></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Column 2: OD Readings at 595nm</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Column 2: OD Readings at 595nm</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><table width="<del class="diffchange diffchange-inline">300px</del>" border="<del class="diffchange diffchange-inline">0.5</del>"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><table width="<ins class="diffchange diffchange-inline">200px</ins>" border="<ins class="diffchange diffchange-inline">1</ins>"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><tr> <th><del class="diffchange diffchange-inline">[</del>CPR<del class="diffchange diffchange-inline">]</del>(mM)</th> <th><del class="diffchange diffchange-inline">OD </del>Reading</th></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><tr> <th>CPR <ins class="diffchange diffchange-inline">Concentration</ins>(mM)</th> <th><ins class="diffchange diffchange-inline">Optical Density </ins>Reading</th></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr> <td>0.25</td> <td>0.021</td> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr> <td>0.25</td> <td>0.021</td> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr> <td>0.50</td> <td>0.033</td> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr> <td>0.50</td> <td>0.033</td> </tr></div></td></tr>
</table>Sj.dixon