Team:Berkeley

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<div id="top-section2"> <p>UC BERKELEY</p> </div>
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    <li><a href="https://2011.igem.org/Team:Berkeley">Home  </a></li>
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        <a href="https://2011.igem.org/Team:Berkeley/Project">Project</a>
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        <a href="https://2011.igem.org/Team:Berkeley/Parts">Parts</a>
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    <li><a href="https://2011.igem.org/Team:Berkeley/Safety">Safety</a></li>
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  <div class="col7" id="content1" ><h2> Project Summary: Stress Promoters </h2> <p> Our team is working on characterizing and manipulating promoters to respond to stress in order to curtail the expression of certain toxic proteins. The promoter sits in front of the toxic protein and will shut off in response to cellular stress caused by that protein. In affect creating a negative feedback system that will allow the protein to be expressed at its maximum level without causing harmful cellular stress.</p></div>
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  <div class="col5" > We are currently in the process of updating our website. </div>
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<p>Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors. </p>
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<p>'''Would any of your project ideas raise safety issues in terms of:'''
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** researcher safety,
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** public safety, or
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** environmental safety?
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Nothing we're working on has any special safety concerns. Working with E.Coli generally poses some safety hazards but proper handling avoids any risk factors for researchers, the public, or the environment. <br>
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'''Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,'''
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<div class="col3-2"style="background-color:#101a4d;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxR"> <img src="https://static.igem.org/mediawiki/igem.org/1/10/Subtitlepic1.jpg"
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** did you document these issues in the Registry?
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** how could other teams learn from your experience?
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<p style="text-align:center; color:#CECECE;"> A protein with great potential as a general biosensor system.</p> </div>
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We are working with ToxR which borders on being an RG2 part since it is part of a virulence island, but as a transcription factor is shouldn't be considered dangerous. Other than that, nothing we are working with raises any safety concerns. <br>
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<div class="col3-2"style="background-color:#185f73;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxRChimera"><img src="https://static.igem.org/mediawiki/2011/b/bb/Subtitlepic3header.jpg"
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<p style="text-align:center; color:#CECECE;"> Chimeric proteins that drive translation off of the Pctx promoter.</p></div>
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<div class="col3-2" style="background-color:#303285"><a href="https://2011.igem.org/Team:Berkeley/Project#StressPromoters"><img src="https://static.igem.org/mediawiki/2011/2/2b/Subtitlepic2header.jpg"
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<p style="text-align:center; color:#CECECE;"> Our method for expressing interesting (but toxic) proteins.</p> </div>
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'''Is there a local biosafety group, committee, or review board at your institution?'''
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<div class="col3-2"style="background-color:#499394"><a href="https://2011.igem.org/Team:Berkeley/Project#EstrogenBiosensor"><img src="https://static.igem.org/mediawiki/2011/c/c8/Subtitlepic4.jpg"
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** If yes, what does your local biosafety group think about your project?
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<p style="text-align:center; color:#CECECE;"> Bacteria engineered to detect the presence of estrogen. <br></p></div>
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There are no special safety issues raised by this work beyond the general use of E. coli and recombinant DNA. <br>
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'''Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?'''
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Managing biosafety with new software tools by integrating safety into design would improve the general safety of bacterial engineering.<br>
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<img src="https://static.igem.org/mediawiki/2011/b/bd/Aboutus.jpg">
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<p>We are Team Berkeley, a cohesive unit of 7 undergraduates and 3 advisers. Earlier this year we planned a complex and risky project, given the short amount of time iGEM made available. We quickly learned each others strengths and weaknesses and developed standard systems of organizational management in order to synchronize our efforts for the many parallel tasks at hand. We created protocols, shared them with one another, and worked together on troubleshooting. Using google docs to keep up with the status of cloning projects, the results of assays, material logistics, or the final steps required to complete a project ensured that through the months of hard work, we fine-tuned our ability to work together. As a team, we have learned firsthand how the synthetic biology community relies on the goal-oriented cooperation of skilled individuals from very different backgrounds and with very different skill sets. Some of us have strong engineering backgrounds while others of us have strong biology backgrounds, but we all share a desire to build synthetic biological systems that solve human problems. We are proud of the project that we have created which we will present at the Jamboree together in October.</p>
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<p style="text-align:left; color:#CECECE; font-size:13px; font-weight:bold; padding:3px"> The UC Berkeley iGEM team would like to thank Autodesk and Agilent for their financial support and Synberc, for their administrative support. </p>
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Latest revision as of 03:39, 29 September 2011

Berkeley iGEM 2011

header
Mercury

Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors.

A protein with great potential as a general biosensor system.

Chimeric proteins that drive translation off of the Pctx promoter.

Our method for expressing interesting (but toxic) proteins.

Bacteria engineered to detect the presence of estrogen.


We are Team Berkeley, a cohesive unit of 7 undergraduates and 3 advisers. Earlier this year we planned a complex and risky project, given the short amount of time iGEM made available. We quickly learned each others strengths and weaknesses and developed standard systems of organizational management in order to synchronize our efforts for the many parallel tasks at hand. We created protocols, shared them with one another, and worked together on troubleshooting. Using google docs to keep up with the status of cloning projects, the results of assays, material logistics, or the final steps required to complete a project ensured that through the months of hard work, we fine-tuned our ability to work together. As a team, we have learned firsthand how the synthetic biology community relies on the goal-oriented cooperation of skilled individuals from very different backgrounds and with very different skill sets. Some of us have strong engineering backgrounds while others of us have strong biology backgrounds, but we all share a desire to build synthetic biological systems that solve human problems. We are proud of the project that we have created which we will present at the Jamboree together in October.


The UC Berkeley iGEM team would like to thank Autodesk and Agilent for their financial support and Synberc, for their administrative support.


Retrieved from "http://2011.igem.org/Team:Berkeley"