Team:Berkeley

From 2011.igem.org

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Revision as of 01:23, 4 September 2011

Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological devices capable of detecting a wide variety of molecules. A periplasmic domain causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved to be toxic to E. coli. To address ToxR toxicity, we screened microarray data for promoters that exhibited stress-based down regulation. We constructed a negative feedback system with the rffGH promoter, which permits the use of potentially toxic proteins like our various ToxR chimeras. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be applied to develop new biosensors.

Etiam consectetur nisi et leo luctus varius non ut purus. Aenean eget viverra velit. Quisque semper consequat purus non euismod. Aenean tortor mi, sollicitudin eget accumsan eget, tincidunt vitae sapien. Curabitur a lorem velit.

The UC Berkeley iGEM team would like to thank Agilent for their financial support and Synberc, for their administrative support.

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+   <div class="col6"  ><img src="https://static.igem.org/mediawiki/2011/1/12/ProjectSummaryHeader.jpg" width="490"> <p> Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological devices capable of detecting a wide variety of molecules. A periplasmic domain causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved to be toxic to E. coli. To address ToxR toxicity, we screened microarray data for promoters that exhibited stress-based down regulation. We constructed a negative feedback system with the rffGH promoter, which permits the use of potentially toxic proteins like our various ToxR chimeras. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be applied to develop new biosensors. </p>
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