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Team:Baltimore/Project
From 2011.igem.org
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Revision as of 17:55, 14 July 2011
| Home | Team | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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TEAM BALTIMORE'S ABSTRACT
Our Goal is to provide a way for anyone to be able to make their own Taq polymerase instead of having to purchase it. However, the gene for Taq polymerase has a PstI site located within the coding sequence which makes it incompatible with the BioBrick assembly standard. In order to make the Taq polymerase compatible with the BioBrick assembly standard we need to change the sequence through site directed mutagenesis in a way that would maintain the amino acid sequence of the protein but change the DNA sequence of the gene.
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