Team:BYU Provo/Team Thermosensor/Week23

From 2011.igem.org

Team BYU Provo

BYU Provo
 

Week 23 (Sep 18 - 23)

9/19/11

  • Restreaked all the colonies onto new ara/amp/Xgal plates to redo the experiment one last time. Placed in 30ÂșC incubator
  • Took colonies that looked promising and made overnights to do plasmid preps.

Examples of these plates that were double checked.

  • IGM45, our wild-type thermosensor is in upper left quadrant (9 o'clock-10 o'clock space).
IGM45 vs. Hour 1 thermosensors, after experimentation
IGM45 vs. Hour 5 thermosensors, after experimentation
IGM45 vs. Hour 8 thermosensors, after experimentation

9/20/11

  • Plasmid prep on 14 of the colonies to sequence our thermosensor